摘要
根据离子选择电极稀释法（间接法）的基本原理，研制出测定Ｎａ、Ｋ、Ｃｌ、ＣＯ＿２的全套试剂，并在ＳＹＮＣＨＲＯＮＥＬ－ＩＳＥ分析仪上，将自制全套电解质测定试剂与ＢＥＣＫＭＡＮ原装试剂进行了对照研究。结果表明自制试剂测定的重复性好，准确性高，与进口试剂相关良好（ｒ＿（Ｎａ）＝０．９９８，ｒ＿Ｋ＝０．９９９，ｒ＿（Ｃｌ）＝０．９９８，ｒ＿（ＣＯ＿２）＝０．８８９）；批内重复性，ＣＶ％＜３．３；批间重复性，ＣＶ％＜５．７；回收率９９％～１０５％。通过对电极斜率和ＳＰＡＮ值的观察表明，自制试剂对电极无影响。
Based on the principle of the Ion Selected Electrode dilution method(indirect),a complete set of reagents for determining Na,K,Cl and CO2 have been developed.Compared with original BECKMAN reagents being applied in the SYNCHRON EL-ISE analyzer,the self-made reagents may satisfy clinical requirements for precision and accuracy,and have a good correlation with original reagents:rNa=0.998,rK=0.999,TCl=0.998,rCO2=0.889,and the CV% within and between batches are less than 3.3 and 5.7 respectively;the rate ofrecovery is 99%～105%. NO influence on the electrode has been found through observing electrode slopes and SPAN values.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1996年第4期301-304,共4页
Acta Academiae Medicinae Sinicae
