摘要
为了探讨极低频（ＥＬＦ）磁场可能存有的促癌作用或协同促癌作用，观察了５０Ｈｚ磁场对ＣＨＬ细胞间缝隙连接通讯（ＧＪＩＣ）功能的影响。利用离子电渗注射法将荧光染料引入细胞内，以５分钟后的荧光偶合细胞（ＤＣＣ）数作为ＧＪＩＣ功能的指标。研究了不同浓度的十四酰基咐拜醇酯（ＴＰＡ）、不同强度的工频磁场及工频磁场与ＴＰＡ（５ｎｇ／ｍｌ）共同作用对ＣＨＬ细胞ＧＪＩＣ的影响。结果表明，ＴＰＡ对ＧＪＩＣ的抑制具有浓度依赖关系，ＴＰＡ抑制ＣＨＬ细胞ＧＪＩＣ的阈浓度在１～５ｎｇ／ｍｌ之间；ＣＨＬ细胞经０.８０ｍＴ的工频磁场暴露２４小时后的ＤＣＣ数降至６．０８±１．５９，与空白对照组（９．８４±２．２７）比较，差异有显著性（Ｐ＜０．０５）；０．２０，０．４０，０．８０ｍＴ强度的工频磁场与５ｎｇ／ｍｌＴＰＡ共同作用后的ＤＣＣ数分别降至５．５２±１．５３，５．００±１．２２，４．００±１．２９，与单用５ｎｇ／ｍｌＴＰＡ组（６．３８±１．３９）比较，差异也有显著性（Ｐ＜０．０５）。说明一定强度的工频磁场具有抑制或协同抑制ＧＪＩＣ的作用，提示其可能存有促癌或协同促癌作用。
In order to explore if the extremely low frequency(ELF)magnetic field can act as cancerpromoter or be synergistic with TPA in cancer promotion,the effect of 50 Hz magnetic field exposureand TPA on gap junctional intercellular communication(GJIC) of CHL cells was observed. Lucifer dyewas led into CHL cells by iontophoretic injection and the nurnber of dye-coupled cells(DCC)five min-utes after injection was adopted as the index of GJIC, The results showed that the suppression of TPAon GJIC was dependent on its concentrations and the threshold concentration of TPA was between 1～5ngm/ml;after exposure to 0.80 mT magnetic field for 24 hours,the number of DCC decreased to 6. 08±1. 59 whereas that in control group was 9.84±2.27(P<0. 05);when 5 ng/ml TPA treatrnent combinedwith exposure to 0.20,0.40 and0.80 mT magnetic field respectively,the number of DCC was 5.52±1. 53,5. 00±1. 22,and 4. 00±1. 29 respectively,whereas that in the group treated with 5 ng/ml TPAaIone was 6. 38± 1.39(P<0.05).It is suggested that certain intensities of 50 Hz magnetic field expo-sure might act as cancer promoter or be synergistic with other cancer promoters.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
1996年第4期210-212,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家和浙江省自然科学基金
关键词
工频磁场
GJIC
细胞
缝隙连接通讯
致癌性
Power frequency magnetic field Gap junctional intercellular communication Cancer promotton
