摘要
目的:研究脊髓缺血再灌注早期脊髓组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性变化,以探讨自由基在脊髓继发性损伤中的作用。方法:36只成年雄性SD大鼠采用腹主动脉肾下夹闭法造成脊髓缺血再灌注模型,连续观察再灌注后1 h4、h、8 h1、6 h2、4 h脊髓组织内MDA和SOD活性。结果:脊髓缺血再灌注后脊髓组织MDA立即上升,8 h达最高峰,此后随之下降,但至24 h仍高于正常对照组;SOD活性显著下降,8 h达最低,随后逐渐回升,至24 h基本接近缺血前。结论:MDA和SOD参与了脊髓缺血再灌注继发性损伤的病理过程。
Objective： To investigate the early dynamic changes of free radical and superoxide dismutas（SOD） after spinal cord ischemia reperfusion associated with abdominal aortic cross - damping and explore mechanism of ischemia reperfusion injury. Methods： 36 adult male Sprague - Dawley rats were divided randomly into control group and ischemia reperfusion groups. The concentration of malonyldialdehyed（MDA） and activities of SOD in spinal cord of these animals were measured at pre - ischemia, reperfusion 1 h, reperfusion 4 h, reperfusion 8 h, reperfusion 16 h and reperfusion 24 h, respectively. Results： The concentration of MDA increased irnmediately after reperfusion, reached to peak within 8 h and then began to decrease, but it still was higher than that of the control group. The activity of SOD decreased during reperfusion, the lowest point was present at 8 h and similar to that of the control group. Condusion： It is suggested that free radical plays an important role in the secondary pathological injury of spinal cord induced by ischemia reperfusion.
出处
《西南国防医药》
CAS
2005年第6期591-593,共3页
Medical Journal of National Defending Forces in Southwest China
关键词
缺血再灌注
自由基
超氧化物歧化酶
动物
实验
ischemia reperfusion, free radical, superoxide dismutas, injury, rat
