摘要
目的:建立反相高效液相色谱法测定食品中的苏丹红1号的方法.方法:食品中的苏丹红1号经乙醇提取后用反相高效液相色谱一二极管阵列检测器(DAD)测定.结果:该方法线性范围从0.5～1000μg/ml,标准差为0.18,相对标准偏差为0.73%,平均回收率为95.43%,最低检出限为0.2ng,最低检出浓度为0.02 mg/kg.人工合成色素(柠檬黄、日落黄、胭脂红、苋菜红、亮蓝、靛蓝、靛蓝)不干扰其测定.结论:该方法线性范围广、精密度高、准确度好、选择性强,完全能满足食品中苏丹红1号的定性定量分析.
Objective： To evaluate the method of detection of Sudan I in foods based on the use of RE- HPLC. Methods： Sudan 1 in foods were detected by re- FIPLC- DAD after extracted by alcohol. Results： This method could detect Sudan I in samples effectively while its concentration varied from 0.5 μg/ml to 1 000 μg/ml. The standard deviation was 0.18, relative standard deviation was 0.73%, and average recovery was 94.43%. 0.2 ng was observed as its detection limit, while the concentration limit was 0.2 mg/Kg. Artificial synthesized pigments had no disturbance in the process. Conclusion： This method is validated in terms of detection limits, linearity, sensitivity, accuracy and selectivity in detection of Sudan I in foods.
出处
《现代预防医学》
CAS
北大核心
2005年第12期1760-1761,共2页
Modern Preventive Medicine
