摘要
用操作性条件反射的方法观察Ｄ－半乳糖亚急性中毒大鼠学习记忆行为的影响。将Ｗｉｓｔａｒ大鼠进行１ｗ的操作性条件反射训练，达到稳定操作状态后，将其分为对照组和实验组，实验组再分为３个半乳糖剂量组，分别用Ｄ－半乳糖１２ｍｇ，２４ｍｇ，和４８ｍｇ·ｋｇ－１·ｄ－１，ｓｃ，共６ｗ。各组均为６只大鼠。用药期间，每天进行了３０ｍｉｎ操作行为ＦＲ４的测试。记录操作潜伏期，操作比率及３０ｍｉｎ的辨别能力测试。结果显示，用药３ｗ后，各剂量组大鼠的操作比率明显下降，潜伏期明显延长（Ｐ＜０．０１），辨别性操作能力逐渐下降。上述改变以４８ｍｇ·ｋｇ－１剂量组最为显著。这提示，Ｄ－半乳糖亚急性中毒状态下能干扰大鼠的学习记忆的巩固、再现和辨别能力，这种学习记忆的损害与文献报道的Ｄ－半乳糖诱导的拟衰老变化是一致的。
The learning and memory impairment were measured by using microcomouter-controlled operant apparatus in aging rat model induced by D-galactose.D-galactose after being administered for six weeks,the mimetic aging model was established and it's effects on fixed ration schedule-controlled behavior were observed.It was found that the latent period of operation was remarkably prolonged(32±10 sec)comparing with controlled ones (2±1 sec),and the behavior of pressing the lever was disrupted and the rate of the response was reduced. Discrimination was also observed in the experiment and the D-galactostreated rats showed a lower ahility to discriminate.The significance of establishing aging-like rat model with memory and learning deficit was discussed.
出处
《中国老年学杂志》
CAS
CSCD
1996年第4期230-232,共3页
Chinese Journal of Gerontology
关键词
衰老
D-半乳糖
学习记忆障碍
操作性条件反射
D-galactose
Aging model
Learning and memory dysfunction
Operational conditional reflex
