摘要
建立了快速测定果蔬产品中阿维菌素及其有毒代谢物残留量的柱前衍生高效液相色谱测定方法。果蔬样品经乙腈提取后,直接采用三氟乙酸酐(TFAA)-N-甲基咪唑(NMIM)-乙腈(ACN)法进行柱前荧光衍生化,后以高效液相色谱-荧光检测器进行阿维菌素残留分析。试验结果表明,本方法对果蔬产品中的阿维菌素及其有毒代谢物残留量的最低检测浓度为1μg·kg-1,在0.001～0.1mg·kg-1时,阿维菌素的添加回收率在84.2%～110.8%之间,变异系数(C.V.%)在1.9%～12.8%。
A rapid HPLC method for simultaneous determination of abamectin and its toxicological metabolite in vegetables and fruits with fluorescent detector after pre-column derivatization was developed. After extracted by acetonitrile, overleaping cleaned-up step,the target compounds in samples was directly pre-column derivatised by TFAA-NMIM-CAN system. The results suggested that the fortified recovery and variation coefficient of added compouds in samples were 84.2%- 110.8% and 1.9%- 12.8%, respectively, the limited level of detection is 1 μg·kg^-1.
出处
《中国农业科学》
CAS
CSCD
北大核心
2005年第11期2254-2260,共7页
Scientia Agricultura Sinica
基金
上海市科技兴农重点攻关项目(沪农科攻字2003第10-6号)
