摘要
用魔芋多糖(KGM)将肌红蛋白(Mb)固定在玻碳电极表面,制备了Mb-KGM膜修饰电极.包埋在KGM膜中的肌红蛋白与电极直接传递电子.在-0.2V～0.8V循环扫描,可得一对可逆氧化还原峰,式电势为-0.403V,这是肌红蛋白辅基血红素FeIII/FeII电对的氧化还原.其式电势随溶液pH值增加而负移且呈线性关系,直线斜率为-47.6mV/pH,说明肌红蛋白的电子传递过程伴随有质子的转移.研究了Mb-KGM膜修饰电极对O2、H2O2、NO电催化性质.
Myoglobin （Mb）, immobilized on glassy carbon electrode （GCE） surface by konjac glucomannan （KGM） hydrogel, can directly transfer electron with GCE. A pair of redox peaks with the formal potrntial -0.403 V vs SCE corresponding to MbFe^Ⅲ/Fe^Ⅱ couple was observed in pH7.0 PBS buffer. The formal potential of MbFe^Ⅲ/Fe^Ⅱ couple shifted negatively and linearly with a decrease ofpH. The value of slope of is -47.6mV/pH, which suggests that one proton transfer in the one-electron reaction. Mb entrapped in KGM film exhibits the electrocatalytic activity to oxygen, hydrogen peroxide and nitric oxide. For H2O2 the cathodic peak current increase linearly with the concentration of H2O2 in the range of 16.68-169.45μmol·L^-1; For NO2^-, and the cathodic peak current is linear proportional to the concentration of NO2^- in the range of 0.70-5.10 mmol·L^-1.
出处
《襄樊学院学报》
2005年第5期32-36,共5页
Journal of Xiangfan University
基金
湖北省教育厅科学技术研究项目(G200525001)
关键词
肌红蛋白
魔芋多糖
直接电化学
电催化
Myoglobin
Konjacglucomannan
Direct electrochemistry
Electrocatalysis
