摘要
目的：探讨血管活性肠肽(VIP)对感染性休克大鼠肠道的保护作用及其机制。方法：采用盲肠结扎加穿 孔(CLP)法制备感染性休克模型。30只健康 SD 大鼠随机分成3组：对照组(假手术组，SO)、CLP 组和 VIP 组。SO 组和 CLP 组术后经股静脉注射生理盐水，VIP 组则注射 V1P 5nmol，持续监测平均动脉压(MAP)、采集血样，采用酶联免 疫吸附试验(ELISA)检测血清肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10) 水平，并取小肠标本行病理和电镜检查。结 果：与对照组比较，CLP 组术后 MAP 进行性下降，血清 TNF-α、IL-10水平升高，显微镜和电镜下小肠有明显的病理改 变。VIP 能有效阻止 MAP 的下降，降低血清 TNF-α和提高 IL-10水平，显微镜和电镜下小肠的病理损害明显改善。结 论：VIP 对感染性休克大鼠及其小肠结构和功能有明显的保护作用，其机制可能与影响细胞因子的产生有关。
Objective To investigate the protective effects of vasoactive intestinal peptide （VIP） on intestine of septic shock rats and explore its possible mechanism. Methods Cecal ligation and puncture （CLP） were used to reproduce septic shock model. Thirty healthy, adult Sprague-Dawley rats were randomly divided into 3 groups of 10 animals each： sham operation（SO） group, CLP group and VIP group. The rats in SO and CLP groups received intravenous injection of normal saline, and VIP was infused in the VIP group with a dosage of 5 nmol per rat. Mean arterial pressure （MAP） was monitored consistently, and blood samples were taken from the femoral artery for measuring the serum concentrations of tumor necrosis factor-α（TNF-α） and interleukin-10 （IL-10） by using enzyme-linked immunosorbent assays（ELISA）. Intestinal samples were harvested for pathological and electroscopic examination. Results Compared with the SO rats, the CLP rats produced progressive hypotension, and a large increase in serum TNF-α and IL-10 concentrations, with obvious pathological injuries in the intestine. In the VIP group rats, the hemodynamic responses were reversed, and serum TNF-α concentration was suppressed meanwhile IL-10 level was increased with markedly alleviated intestinal pathological injuries. Conclusions VIP exerts protective effects on septic shock rats by inhibiting the production of proinflammatory factors and by stimulating the production of anti-inflammatory cytokines, which might be used as an alternative therapy for septic shock.
出处
《外科理论与实践》
2005年第6期546-548,共3页
Journal of Surgery Concepts & Practice
