摘要
研究目的:研究运动训练后采用低氧处理对肾组织低氧应激反应基因mRNA表达的影响。研究方法:将健康雄性SD大鼠32只,随机分为4组,采用基因芯片方法,检测肾组织中低氧应激反应基因mRNA表达。研究结果:低氧训练可改变大鼠肾组织中部分低氧应激基因的mRNA的丰度,其中,PDIPLmRNA、RHODANESEmRNA表达上调,AMmRNA、HSP40mRNA、ECE1mRNA、ENO1mRNA、IL1BmRNA、IL6mRNA、TSG14mRNA表达下调;低氧训练没有改变IL1AmRNA、BMYBmRNA的丰度。肾组织中低氧应激基因mRNA在低氧训练的第3、6、13天呈现不同的变化,RHODANESEmRNA表达持续上升,而AMmRNA、HSP40mRNA、ECE1mRNA、ENO1mRNA、IL1BmRNA、IL6mRNA、PDIPLmRNA、TSG14mRNA表达下调并于训练的第13天回到安静状态的表达水平。
This paper studied the effect of hypoxic training on mRNA expression of stress genes of rat renal tissue. 32 male SD rats were randomly divided into four groups,and gene array method was applied to exam the mRNA expression of stress genes of rat rental tissues. The result showed that hypoxic training could change the contents of some hypoxic stress genes mRNA of rat renal tissue. The expression of PDIPL mRNA, RHODANESE mRNA was upgraded,and the expression of AM mRNA,HSP40 mRNA,ECE1 mRNA ENO-1 mRNA,IL- 1B mRNA, IL-6 mRNA, TSG-14 mRNA was downgraded, and IL-1A mRNA, BMYB mRNA was kept at basic level. Hypoxic stress genes mRNA showed different change pattern on three, six and thirteen day of hypoxic training. RHODANESE mRNA was at high level during hypoxic training comparing with control group. AM mRNA, HSP40 mRNA, ECE1 mRNA, ENO- 1 mRNA, IL-1B mRNA, IL-6 mRNA, PDIPL mRNA, TSG-14 mRNA were increased after hypoxic training,and return to basic level on thirteen days of hypoxic training.
出处
《体育科学》
CSSCI
北大核心
2005年第12期56-60,共5页
China Sport Science
基金
湖南省科技厅专项课题(02SSY2009)。
关键词
低氧训练
基因芯片
应激反应基因
转录
鼠
动物实验
hypoxic training
gene array
stress gene
transcription
rat
animal experiment
