摘要
通过盆栽实验研究了重金属Cu污染对植物(三叶草)-土壤酶(脲酶、蔗糖酶、过氧化氢酶和多酚氧化酶)系统的影响.结果表明,随着Cu浓度增加,脲酶、蔗糖酶、过氧化氢酶和多酚氧化酶活性均逐渐减小,与Cu浓度有高度相关性,蔗糖酶>多酚氧化酶>脲酶>过氧化氢酶.在处理浓度不变情况下,酶活性随时间而变化,且呈现低Cu浓度(<500mg.kg-1)时4种酶活性均有所上升,而Cu浓度增高(500～3000mg.kg-1)时各酶活性逐渐下降的趋势.统计分析表明,在每一梯度浓度上,4种酶在Ⅰ、Ⅱ、Ⅲ组内均存在显著差异性(P<0.01),与植物受重金属Cu污染时的生长情况一致.随着Cu浓度增加,土壤pH值逐渐下降,而电导率上升;同一Cu浓度下的pH值和电导率均随时间呈缓慢上升趋势,统计分析显示,二者在Ⅰ、Ⅱ、Ⅲ组内均存在显著差异性(P<0.01).土壤pH值和电导率与4种土壤酶活性有高度相关性,多酚氧化酶>蔗糖酶>过氧化氢酶>脲酶.这4种酶同时可作为检测土壤环境质量的指标.
The study with pot experiment showed that with increasing Cu concentration, soil urease, invertase, catalase and polyphenol oxidase activities decreased gradually. There was a significant correlation between Cu concentration and soil enzyme activities, with the correlated degree followed the order of invertase 〉 polyphenol oxidase 〉 urease 〉 catalase. Under a fixed Cu concentration, soil enzyme activities changed with time, and the changes were different between high and low Cu concentrations, being increased slightly under low Cu concentration （ 〈 500 mg·kg^-1 ）, but decreased gradually as Cu concentration increased （ 500 - 3 000 mg·kg^-1）. Statistical analysis indicated that within the range of test Cu concentrations, the activities of test soil enzymes were significantly different among different Cu concentration （ P 〈 0.01 ）, which was accorded with the seedlings growth status. Soil pH was decreased, while electric conductivity was increased with increasing Cu concentration （ 500 - 3 000 mg·kg^-1）, but they were increased with time under a fixed Cu concentration, with significant difference among different Cu concentration （P 〈 0.01 ）. Soil pH and electric conductivity were highly related to soil enzyme activities, with the order of polyphenol oxidase 〉 invertase 〉 catalase 〉 urease. The test soil enzyme activities could be used as the indices of soil environment quality.
出处
《应用生态学报》
CAS
CSCD
北大核心
2005年第12期2413-2417,共5页
Chinese Journal of Applied Ecology
基金
国家重点基础研究发展规划项目(2004CB418503)
国家自然科学基金项目(30470270)
安徽省教育厅自然科学基金项目(2005kj183)
安徽师范大学青年科学基金资助项目(2005xqn11).
关键词
铜
三叶草
生长
土壤酶活性
Cu, Trifolium repens, Growth, Soil enzyme activity.
