摘要
应用聚合酶链反应技术扩增结核杆菌特异的４２０ｂｐＤＮＡ片段靶基因诊断结核病，并以培养法和涂片法作比较。共检测１４５份肺结核病的痰标本，阳性９１份，阳性率达６２．７６％，而培养法和涂片法阳性率仅分别为３８．６２％和１３．１％。但在５６份阳性标本中，有５份培养法和涂片法均为阳性的痰标本，而聚合酶链反应为阴性，假阴性率８．９２％。４２份健康人痰标本未见阳性扩增。
Totol 145 sputum specimens from the patients with pulmonary tuberculosis were tested by Polymerize Chain Reaction(PCR).technique which augmented the specific 420 BP DNA segment targetgene. Comparing PCR with culture and smear method,the positive rate were62.76%、38. 62%and 13.1% respectively. Among 56 positive specimens,there were 5 specimens which were positive by culture and smear method,but negative by PCR. The false negative rate was 8. 92%. No positive augmentation could be found among the 42 sputum specimens of healthy people.
出处
《实用预防医学》
CAS
1996年第2期110-111,共2页
Practical Preventive Medicine
