摘要
以中黄13号大豆种子为材料,取生长8天的健康子叶,在其表面挖出约2×2mm的伤口,将50μl不同浓度的茉莉酸溶液和1mM的硝酸银溶液滴在伤口处,黑暗中(25℃)保温不同时间后取样,经甲醇提取,用高压液相色谱(HPLC)测定其中异戊烯化异黄酮glyceollins及其前体glycinol 的累积情况.结果显示,健康子叶中没有glyceollins和glycinol的累积,1mM硝酸银比茉莉酸具有更强的诱导glyceollins和glycinol累积的能力,glyceollins的累积与茉莉酸浓度呈负相关.在各种处理中,glyceollinⅠ、Ⅱ、Ⅲ所占比例分别为:50%～80%、10%～40%和10%～20%,但是glyceollin三种异构体的比例随保温时间的延长而有所变化,glyceollin Ⅰ比例逐渐下降,glyceollin Ⅱ所占比例逐渐升高,在1mM硝酸银处理的子叶中表现更为明显.
Health cotyledons were detached from 8-day-old soybean seedlings （Zhonghuang No. 13）. A section of tissue, approximately 2×2mm was cut from the concave surface. 50μl jasmonic acid or silver nitrate solution was dripped into the wound and incubation in the dark at 25℃. Glyceollins and glycinol were extracted by methanol and analyzed by HPLC. The results show that there is no accumulation of glyceollins and glycinol in health cotyledons. Silver nitrate acts much stronger than jasmonic acid to induce the accumulation of glyceollins and glycinol. The accumulation of glyceollins is in inverse proportion to the concentration of jasmonic acid. The percent of 3 glyceollin isomer is 50%-80%, 10%-40% and 10%-20% for glyceollin Ⅰ, Ⅱ and Ⅲ, respectively. During the incubation, the ratio of glyceollin Ⅰ decreases and that of glyceollin Ⅱ increases. It can be obviously observed in samples treated by silver nitrate.
出处
《大豆科学》
CAS
CSCD
北大核心
2005年第4期269-274,共6页
Soybean Science
基金
荷兰瓦赫宁根大学“交叉研究和教育基金”项目,项目名称:植物雌激素一有健康保护作用的植物次生代谢物一植物中黄酮异戊烯转移酶基因的分离。
