摘要
０．５ｍｏｌ／ＬＨＣｌ处理ｌｈ后解离ＨＢｓＡｇ一抗Ｈｈ免疫复合物，并变性抗ＨＢｓＡｇ抗体而对ＨＢｓＡｇ免疫反应性无影响，３４例临床抗ＨＢｓ＋血清样品经ＨＣｌ处理前后，常规ＥＬＩＳＡ检测ＨＢＳＡｇ比较，结果：酸处理前ＨＢｓＡｇ阳性率为１１．７６％，处理后增加至４１．１８％（Ｐ＜０．０１），实验表明血清样品经酸处理后能明显提高临床乙肝ＨＢｓＡｇ检出率。
lmproved detection of HBsAg of the human hepatitis B virus in serd was obtainedby 0.5mol/L HCL pretreatment of samples before using a standard HBsAg ELISA. pretreatmentdissociated and denatured HBsAg-anti HBs immune complexs but with Iittle or no compromise ofHBsAg immunoreactivity.In34 anti-HBs+sera, direct comparison of thepretreatmental assay withthe conventional demonstrated that substantial increase in HBsAg positivity in the pretreated assay(11.76%M41.18%).Serum HBsAg is partly in the immune complexs form.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1996年第2期172-174,共3页
Chinese Journal of Pathophysiology
关键词
乙型肝炎
表面抗原
ELISA
Enzyme-linked immunosorbent assay·Hepatitis B surface antigens·Antigens,immmune response
