摘要
从抗人脑胶质瘤单克隆抗体杂交瘤细胞中抽提总ＲＮＡ，并以Ｏｌｉｇｏ（ｄＴ）柱纯化ｍＲＮＡ，经逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）直接扩增出３１８ｂｐ的轻链可变区（ＶＬ）ｃＤＮＡ片断，经核苷酸序列分析研究，证实已获得了ＳＺ－３９单抗轻链可变区基因。这为今后进一步制备基因工程抗体打下基础。
We both have extracted total RNA and purified mRNA by(dT)cellulose in the hybridoma cell of anti-human brain glioma monoclonal antibody and have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin light chain variable-region gene by polymerase chain reaction.The primers incorporate restriction sites that allow the cDNA of the variable domain to be force-cloned for sequencing and expression.The result shows that the length of light chain variable-region gene of this antibody is 318 base pairs. This study based for antibody production with genetic engineering.
出处
《中华神经外科杂志》
CSCD
北大核心
1996年第2期72-74,共3页
Chinese Journal of Neurosurgery
基金
"八五"核工业部攻关课题
关键词
单克隆抗体
可变区基因
胶质瘤
脑肿瘤
Reverse transcriptase-polymerase chain reaction(RT-PCR)Monoclonal antibody Variable-region gene Brain glioma
