摘要
目的观察ＣＤＫＮ２基因产物（Ｐ１６蛋白）在肺癌和癌旁正常组织的表达情况，了解该基因与肺癌的关系。方法采用ＳＰ免疫组织化学方法观察ＣＤＫＮ２基因产物在１３９例肺癌组织的表达情况，并与癌旁正常组织进行对比。结果Ｐ１６蛋白在良、恶性细胞的胞浆中均有表达，癌旁正常支气管粘膜上皮和浆液腺Ｐ１６蛋白的阳性率高于癌组织（Ｐ＜０．０１）。肺癌组织中Ｐ１６蛋白的总阳性率为６１．９％，腺癌的阳性率高于鳞癌和小细胞癌（Ｐ＜０．０１）。在鳞癌和腺癌Ｐ１６蛋白的阳性率随着分化程度的降低而下降，高分化和低分化腺癌之间Ｐ１６阳性率的差异有显著性（Ｐ＜０．０５）。结论Ｐ１６蛋白表达缺失与肺癌的发生有关，且随着分化程度的下降表达缺失增加。
ObjectiveToexaminetheexpressionofP16proteininbronchogeniccarcinomaandnormaltisueadjacenttocarcinomaandtodeterminetherelationshipbetweenthegeneandbron-chogeniccarcinoma.MethodUsingarabbitpolyclonalantibodyagainstP16(N-20),theexpresionofP16proteinwasstudiedimmunohis-tochemicalyonformalinfixed,parafinembeddedsections.Results.ThecytoplasmicP16proteinwasmainlypresntinnormalbronchialepithelium(93.8%),serousgland(92.6%)andalveolarepithelium(71.4%),theexpresionlevelsofP16weresignificantlylowerincar┐cinomatissues(61.9%)whencomparedtolevelsintheirnormalcoun┐terparts(P<0.01).Amongtumors,squamouscelcarcinomasandsmalcelcarcinomasexhibitedlowerP16ex┐presionlevelscomparedwithadenocarcinomas(P<0.01).TheexpresionlevelsofP16werelowerinpoorly┐dif┐ferentiatedadenocarcinomascomparedwiththoseinwel┐differentiatedadenocarcinomas(P<0.05).CinclusionsTheresultssuggestedthatCDKN2genewasatumorsuppressorgene,theinactivationofwhichwereinvolvedinthecarcinogenesisofbronchogeniccarcinomaandimplicatedintumordiferentiation.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1996年第2期81-83,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家"八五"攻关基金
关键词
肺肿瘤
抑癌基因
免疫组化
ngneoplasmsCarcinomaTumorsuppresorgeneImmunohis-tochemistry
