摘要
研究再生障碍性贫血(AA)用环孢素A(CsA)治疗前后和骨髓增生异常综合征(MDS)患者外周血CD4+、CD8+T淋巴细胞培养前后早期激活标志CD69的表达及其意义。将外周血在PHA20μg/ml条件下进行全血细胞培养,于0h和4h分别用双色免疫荧光标记流式细胞仪对CD4+、CD8+T淋巴细胞CD69的表达进行分析。发现PHA刺激前初治SAA和MDS-RA+MDS-RAS患者CD4+、CD8+细胞CD69的表达率增高,CAA与RAEB+RAEB-T患者CD8+细胞CD69的表达率增高;PHA刺激后AA与MDS患者CD4+、CD8+细胞表达CD69明显增强,AA患者CD4+细胞CD69的表达率高于CD8+细胞。CsA治疗后SAA患者PHA刺激前CD4+、CD8+细胞CD69的表达率较治疗前明显减低,CAA患者CD8+细胞CD69的表达率较治疗前明显减低。治疗后AA患者PHA刺激后CD4+、CD8+细胞CD69的表达率较治疗前明显减低。CsA治疗有效的AA患者治疗前PHA刺激前后CD4+、CD8+细胞CD69的表达率明显增高,治疗后明显减低。初治AA患者PHA刺激前后CD4+细胞CD69的表达率均明显高于MDS患者。说明T细胞早期活化及其活化潜能增强,以及产生针对自身造血干/祖细胞的细胞毒效应在AA和MDS发病中起重要作用,CsA能抑制AA患者T细胞的早期激活。
To investigate the expression of the early activation marker of T cells（CD69） on CD4^＋ and CD8^＋ lymphoeytes in peripheral blood in patients with aplastie anemia （AA） and myelodysplastie syndrome（MDS） before and after eyelosporin A（CsA） therapy and the pathophysiologieal significance of the changes in CD69 expression in different T cell populations of both preand post-culture in vitro, the whole blood cell culture procedure was applied to activate T lymphoeytes by PHA（20 μg/ml） in vitro. The expression rates of CD69 on CD4^＋ and CD8^＋ lymphoeytes at 0 h and 4 h after culture were analyzed by two-color flow eytometry. It was found the expression rates of CD69 of CD4^＋ and CD8^＋ cells in patients with newly diagnosed SAA and MDS-RA＋MDSRAS and the expression rates of CD69 on CD8^＋ cells in patients with CAA and RAEB＋RAEB-T increased before PHA stimulation. The expression rates of CD69 of CD4^＋and CD8^＋ cells in patients with AA and MDS elevated significantly after PHA stimulation. CD69 expression on CD4^＋ cells was much higher than that on CD8^＋ cells after stimulation in patients with AA. The expression rates of CD69 of CD4^＋ and CD8^＋ cells in patients with SAA and the expression rates of CD69 of CD8^＋ ceils in patients with CAA after CsA therapy before PHA stimulation decreased remarkably compared with those before therapy. CD69 expression on CD4^＋ cells and CD8^＋ cells after PHA stimulation in patients with AA after CsA therapy decreased significantly compared with those before therapy. CD69 expression on CD4^＋ and CD8^＋ cells before and after PHA stimulation in patients with AA could be cured before CsA therapy increased remarkably and decreased significantly after therapy. CD69 expression on CD4^＋ cells before and after stimulation in patients with newly diagnosed AA were much higher than those in MDS. These results suggested that the increased early activation and activated potentials of T lymphocytes might play a major role in the pathogenesis of AA and MDS. CsA might inhibit T cells early activation in AA.
出处
《现代免疫学》
CAS
CSCD
北大核心
2005年第6期497-501,共5页
Current Immunology
