摘要
目的:通过体外分离培养人新鲜脐带血(Umbilical cord blood,UCB)造血干细胞(Hematopoieticstem cell,HSC),探讨不同细胞因子组合对HSC的扩增作用。方法:采用Ficoll淋巴细胞分离液密度梯度离心法分离UCBHSC,加入不同组合的细胞因子,检测有核细胞(Nuclear cell,NC)总数和CD34+细胞数。结果:未加细胞因子的对照组培养初期细胞即已破裂溶解,加入不同细胞因子组合的实验组的NC细胞总数和CD34+细胞数随着培养时间的延长均呈现不同程度的增加,于第14天达到高峰,而含有细胞因子FL、TPO的组合,NC细胞数和CD34+细胞数均比较高。结论:体外HSC培养,加入细胞因子对细胞增殖有明显的作用,而细胞因子FL、TPO协同作用能比较好的扩增早期造血干细胞。
Objective. To investigate the effects of different cytokines on expansion of hematopoietic stem cell （HSC）by isolating and culturing human umbilical cord blood （UCB）. Methods： The UCBHSC were separated with Ficoll reagent using density gradient centrifugation and cultured in DMEM by adding different cytokines. The total nuclear cell（NC） number and CD34^＋ number were detected. Results ： The cells ruptured and dissoluted without cytokines in control group. Compared with control group, the total NC number and CD34^＋ number with different cytokines combination increased along with the time lasting and peaked at the 14th day. The total NC number and CD34^＋ number were fairly higher in groups with cytokines FL, TPO than other groups. Conclusions： During in vitro HSC culture, cytokines have obvious effects on cell proliferation. The combination of cytokines FL and TPO can expand HSC better.
出处
《承德医学院学报》
2005年第4期297-301,共5页
Journal of Chengde Medical University
