摘要
目的:观察糖尿病鼠动脉组织的形态学改变和基因谱变化。方法:21只SD雄性大鼠随机分为正常组9只和链脲佐菌素诱导糖尿病模型组12只,4周后取其主动脉,HE染色,光镜下观察其形态,并用基因芯片技术分析两组之间差异表达的基因谱。结果:糖尿病组主动脉组织形态学上未见到明显的改变,但筛选出109条有显著差异性表达的基因。结论:短期高糖导致各种基因的异常表达先于组织形态学的变化。
Objective： Observing the morphological alterations of artery and Searching the altered gene expression of artery by DNA microarray in diabetic rats. Methods ：21 male SD rats were randomly divided in normal control group（n= 9） and STZ-induced diabetic group （n = 12）. After 4 weeks, the morphological alterations of aorta were observed using light microscope. The altered gene expression was performed by cDNA microarray. Results： There was no markedly difference in morphological alterations between groups. We had got 109 genes that expressed differently, 87 elevated, 22 depressed. Conclusion： The altered gene expression is prior to the morphological changes of aorta in short term hyperglycemia.
出处
《数理医药学杂志》
2005年第6期527-529,共3页
Journal of Mathematical Medicine
关键词
形态学
基因芯片
血管
糖尿病
morphology
gene chip
artery
diabetes mellitus
