摘要
目的检测乳腺癌组织中E-cadherin的表达及患者外周血微转移的发生,探讨二者的相互关系及临床意义。方法应用逆转录-聚合酶链反应(RT-PCR)分别检测15例乳腺良性肿瘤患者及60例乳腺癌患者外周血CK-20及乳腺组织中E-cadherin mRNA的表达。结果15例乳腺良性肿瘤组织中,均有E-cadherin阳性表达(100%),而60例乳腺癌组织中,E-cadherin阳性表达23例(38.3%),二者差异显著(P<0.01);CK-20在乳腺良性肿瘤患者外周血中未见表达。在60例乳腺癌患者外周血中,检出CK-20阳性表达28例(46.7%),E-cadherin在相应癌组织中的阳性表达率为14.3%(4/28),32例外周血CK-20阴性的乳腺癌病例中,E-cadherin的阳性表达率为59.3%(19/32),二者差异显著(P<0.01)。结论乳腺癌组织中E-cadherin的低表达与外周血高转移率显著相关,提示E-cadherin的表达缺失或低表达可能是导致乳腺癌高转移的因素之一,可作为临床判断预后的参考指标。
Objective To explore the expression of E - cadherin in mammary tumor and its clinical significance as well as the relationship with the circulating tumor cell in blood of the breast cancer patients. Methods E - cadherin mRNA in 15 cases of benign breast disease and 40 cases of breast cancer were detected by reverse transcriptase -polymerase chain reaction（ RT -PCR）. CK20 mRNA was also detected by RT -PCR in blood as an index of circulating tumor cells in breast cancer patients. Results Positive rates of E - cadhefin in benign breast disease and breast cancer were 100% and 38. 3% , respectively. There was static significance between the two groups （ P 〈 0.01）,CK20 mRNA was not detected in blood of patients with benign breast disease,In breast cancer patients,the positive rate was 46.7%,Positive rate of E-cadherin in breast cancer patients with CK20 positive disease and negative disease were 14.3%（4/28/）and 59.3%（19/32/）,respectively,There was a significant difference between the two groups（P〈0.01）,Conclusion Aberrant expression of E-cadherin was closely related with the development of blood metastasis in breast cancer.The expressed deficiency or low expression of E-cadherin may be one of the factors responsible for the high metastasis.It may be a prognostic maker of breast cancer.
出处
《现代肿瘤医学》
CAS
2005年第6期758-760,共3页
Journal of Modern Oncology
