摘要
目的探讨p53、bax抑癌基因在肺非小细胞癌组织及癌旁组织中的差异表达。方法以凝胶上rRNA条带的亮度为依据对后续分析用的起始RNA浓度进行调整;以组成型表达的β-actin基因为外对照,对两类组织来源的起始RNA量进行监测;基因克隆及序列分析来验证所用引物的可靠性;随后采用逆转录-聚合酶链式反应(RT-PCR)的方法对各基因在两类组织中的表达谱进行对比分析,并以Northern杂交加以验证。结果在两类组织来源的起始RNA量基本一致的前提下,p53、bax 2个抑癌基因在成对组织中的表达谱存在明显差异。bax在肺癌组织及癌旁组织中均有表达;p53基因在肺癌组织中的表达量较高,且p53的带型与在癌旁组织中表现也明显不同。结论肺癌组织及癌旁组织中存在bax促凋亡基因的转录;p53基因在肺癌组织出现异常转录。
Objective To study the different expression of anti oncogenes p53, bax in lung cancer tissues and its paracancer tissues from different patients. Methods The start concentration of all RNAs were adjusted for the consequent use according to the brightness of rRNA bands displayed on agarose gel. Constitutive expression of B - actin gene was used as external control to monitor the beginning amounts of RNAs from two kinds of tissues. The reliability of all primers used for RT - PCR was confirmed by gene cloning and sequencing. The expression profiles of two genes were then analyzed in tissue parallel by applying RT - PCR. The results were further confirmed by Northern Blot. Results By using similar amount of initial RNAs, two cantioneo genes were differently expressed in the two kinds of tissues, bax were expressed both in lung cancer tissues and paracancer tissues, while p53 exhibited higher expression in lung cancer tissues than in paracancer tissues. Furthermore, the band profile of p53 in lung cancer tissue was sharply different from that in the paracancer tissues. Conclusion The proapoptosis gene bax was unexpectedly tranScribed in lung cancer tissues, suggesting the less effect on the cancer cure by simple apoptosis - promoting method. The abnormal transcription of p53 gene in lung cancer tissue may play an important role on cancer development.
出处
《现代肿瘤医学》
CAS
2005年第6期724-727,共4页
Journal of Modern Oncology
基金
军队医药卫生十五科研基金资助课题(编号01MA101)
