摘要
AIM： To observe the status of tumor-associated B7 molecule mRNA expression in human colorectal cancer tissue by/n situ hybridization. METHODS： The mRNA expression patterns of cancer- associated B7-1,B7H1t,B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization （ISH） with digoxin-labeled oligonucleotide probes. RESULTS： B7-1, B7H1,B7H2,ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes （TIL）. The mRNA expression level of these molecules in tumor cells was higher than that in TIL （0.76±0.54-1.62±0.82 vs 0.38±0.19-0.65±0.33, P〈0.001）. There was no relationship between expression level of tested B7 family molecules and patients＇ sex, age, differentiation status of cancer and regional lymph node metastasis. CONCLUSION： Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1t B7H2 co-signal molecules in tumor cells and TIL. 2005 The WJG Press and Elsevier Inc. All rights reserved
AIM: To observe the status of tumor-associated B7molecule mRNA expression in human colorectal cancer tissue by in situ hybridization.METHODS: The mRNA expression patterns of cancerassociated B7-1, B7H1, B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization (ISH) with digoxin-labeled oligonucleotide probes.RESULTS: B7-1, B7H1,B7H2, ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes (TIL).The mRNA expression level of these molecules in tumor cells was higher than that in TIL (0.76±0.54-1.62±0.82vs 0.38±0.19-0.65±0.33, P＜0.001). There was no relationship between expression level of tested B7 family molecules and patients' sex, age, differentiation status of cancer and regional lymph node metastasis.CONCLUSION: Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1,B7H2 co-signal molecules in tumor cells and TIL.
基金
Supported by the National Natural Science Foundation of China, No. 30370549
