摘要
目的探讨神经系统副肿瘤性综合症(paraneoplastic syndromes of the nerves system,PNSNS)患者体内特异性神经元抗体,发现宿主体内的癌灶。方法收集686例可疑患者的血清或脑脊液,正常人血清50例,采用亲和免疫组织化学法及免疫印迹技术检测其特异性神经元抗体。结果检测患者标本686份,两种方法检测结果如下,亲和免疫组织化学法:抗Hu抗体阳94例、抗Yo抗体阳性3例、抗Ri抗体4例,阳性率14.7%,其中Hu、Yo、Ri抗体分别占13.7%、0.4%、0.6%。免疫印迹技术:抗Hu抗体81例,抗Yo抗体阳性3例,抗Ri抗体4例,阳性率12.8%,其中Hu、Yo、Ri阳性抗体分别占11.8%、0.4%、0.6%,两种方法无显著差异(χ2=1.0370,P>0.05)。正常人血清标本50例,检测其特异性神经元抗体均为阴性。结论检测PNSNS患者体内特异性神经元抗体对发现宿主体内原发癌灶有非常重要的意义。
Objective To probe into specific neuron antibody in patients with paraneoplastic syndrome of the nerver system（PNSNS） and discover the cancer present in their bodies. Methods The serum and cerebral spinal fluid（CSF） specific neuron- antibodies in 686 patients with PNSNS were detected with indirect immunohistochemistory and western blot technique. Results The serum specifity neuron - antibodies in 686 patients with PNSNS and 50 normal people were detected with indirect immunohistochemistory method： 94 showed anti- Hu antibody positive, 3 anti-Yo antibody positive, and 4 anti - Ri antibody positive. The total positive rate was 14.7% ,the positive rate of anti - Hu, Yo and Ri was 13.7% ,0.4% and 0.6%, respectively. The western blot method obtained results as：8l anti- Hu antibody were positive, 3 anti- Yo antibody positive, and 4 anti - Ri antibody positive, the total positive rate was 12.8 %, the positive rate of anti - Hu, Yo, Ri was 11.8%, 0.4% and 0.6%, respectively. The two methods showed no significant statistical difference. （X^2 = 1. 0370, P 〉 0.05）. The serum specific neuron- antibodies in 50 normal subjects were negative. Conclusions It is important to detect specific neuron- antibody in patients with PNSNS to discover the presence of cancer.
出处
《实用预防医学》
CAS
2005年第5期1062-1064,共3页
Practical Preventive Medicine
