摘要
目的探讨离体兔心经冠状静脉窦逆灌心脏停跳与不停跳对心肌细胞膜脂流动性及其功能的影响.方法健康成年家兔24只,体重3.0～3.5kg,随机分为3组:A组(对照组),B组(常温氧合血逆灌心脏不停跳),C组(温血心脏麻痹液持续逆灌),离体兔心顺灌左心做功20min,持续逆灌60min,左心做功60min.比较逆灌后各组心肌细胞膜丙二醛(MDA)含量、心肌细胞膜ATP酶活性、心肌细胞膜脂流动性、心肌细胞内钙离子浓度的变化.结果 B、C两组心肌细胞膜ATP酶活性和心肌细胞膜脂流动性均较逆灌前低(P<0.05),心肌细胞膜MDA含量和心肌细胞内钙离子浓度较逆灌前高(P<0.01).逆灌后,B组心肌细胞膜ATP酶活性和心肌细胞膜脂流动性优于C组(P<0.01),B组心肌细胞膜MDA含量和心肌细胞内钙离子浓度明显较C组高(P<0.01).结论常温氧合血逆灌心脏不停跳在减轻心肌细胞膜自由基损伤,维持膜脂流动性和膜ATP酶功能,减轻细胞内钙超载方面优于温血心脏麻痹液逆灌停搏.
Objective To study the mechanism of myocardial cells membrane damage following retrograde sinus coronary perfusion. Methods Twenty-four isolated rabbits were randomly divided into three groups. Group A received antegrade perfusion. Group B were maitainted heart beating by retrograde perfusion with normothermic oxygenated blood. Group C received retrograde coronary sinus perfusion with warm blood eardioplegia. After 60min retrograde perfusion, the hearts were switched on Langendorff-Neely antegrade perfused working heart model for another 60min. Intramyocyte free calcium, myocardial membrane MDA, ATPase and fluidity were measured. Results After retrograde coronary sinus perfusion, activity of ATPase and fluidity of the myocardial cells membrane in group B was superior to those of group C. The content of myocardial cells membrane MDA and the intramyocyte calcium followed retrograde perfusion was much lower in group B than those of group C. Conclusion Retrograde perfused beating heart was superior to those perfused with warm blood cardioplegia in reducing free radical production, ameliating intramyocyte paradoxical calcium accumulation, and maintaining activity of ATPase and fluidity of the myocardial cells membrane.
出处
《重庆医学》
CAS
CSCD
2005年第11期1655-1656,共2页
Chongqing medicine
关键词
逆灌
心肌细胞膜
功能
细胞内钙
retrograde perfusion
myocardial cellular membrana
function, intramyocyte calcium, beating heart
membrane damage
