摘要
目的:探讨马拉色菌标准株和临床分离株对酮康唑、伊曲康唑和氟康唑的体外敏感性。方法:用生理生化及形态学方法对临床分离的马拉色菌菌株进行菌种鉴定。参照美国临床实验室标准委员会(NCCLS)制订的M27-A方案中酵母菌微量稀释法,检测7种马拉色菌标准株和42株临床分离株对酮康唑、伊曲康唑和氟康唑的最小抑菌浓度(MIC)。结果:42株马拉色菌临床分离株包括5个种,分别是合轴马拉色菌、糠秕马拉色菌、钝形马拉色菌、球形马拉色菌、限制性马拉色菌。用M27-A方案中酵母菌微量稀释法测定3种唑类抗真菌药对7种马拉色菌标准株和42株临床分离株的敏感性由高到低分别为:伊曲康唑、酮康唑、氟康唑。结论:马拉色菌属的各菌种对酮康唑、伊曲康唑和氟康唑的敏感性存在差异;M27-A方案中酵母菌微量稀释法可用于马拉色菌的体外药敏试验。
Objective： To evaluate the in vitro activities of ketoconazole, itraconazole and fluconazole against 7 standard strains and 42 clinical isolates of Malassezia species. Methods： The isolated colonies of Malassezia were identified according to their biochemical, physiological and morphological characteristics. In vitro activities of ketoconazole, itraconazole and fluconazole against 7 standard strains and 42 clinical isolates of Malassezia species were tested by M27-A microdilution method recom mended by National Committee for Clinical Laboratory Standards （NCCLS）. Results： Five species,including M. sympodialis, M. fur fur, M. obtusa, M. globosa, and M. restricta, were identified out of 42 clinical isolates of Malassezia. It was found that the order of 3 azole antifungal agents from high to low susceptibility to Malassezia species was itraconazole〉 ketoconazole〉fluconazole. Conclusion： The susceptibilities of Malassezia species to ketoconazole, itraconazole , and fluconazole are different. Microdilution method from M27-A is suitable for testing the susceptibility of Malassezia in vitro.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第11期1277-1279,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30471566)
关键词
马拉色菌
抗真菌药
微生物敏感性试验
Malasssezia
antifungal agents
microbial sensitivity tests
