摘要
目的：研究一种拟青霉代谢产物提取物(bioactive compounds from paecilomyces tenuipes，BCPT)对慢性应激抑郁大鼠血皮质酮(CORT)、促肾上腺皮质激素(ACTH)含量及下丘脑促肾上腺皮质激素释放激素(CRH)mRNA表达的影响。方法：在慢性温和性不可预见性应激(CUMS)模型上，采用糖水消耗量法观测动物的奖赏反应；以放射免疫分析方法(RIA)测定血清CORT及血浆ACTH含量；利用半定量逆转录．聚合酶链反应(RT-PCR)方法，测定下丘脑CRHmRNA的表达。结果：BCPT 40、80 mg·kg^(-1)可不同程度增加模型大鼠糖水消耗量，改善模型大鼠的奖赏反应，并能不同程度降低模型大鼠血清CORT、血浆ACTH含量；下调慢性应激大鼠下丘脑CRHmRNA的过度表达。结论：BCPT的抗抑郁作用可能与其改善慢性应激大鼠下丘脑-垂体-肾上腺轴(HPAA)功能亢进有关。
AIM： To study the effect of bioactive compounds from paecilomyces tenuipes （BCPT） on the blood level of CORT, ACTH and expression of CPHI mRNA of hypothalamus in chronic unpredictable mild stress model （CUMS） of depression in rats. METHODS ： The depression animal model was induced by CUMS. The reward reaction was tested by the sweet water consumption＋The effect of BCPT on the blood level of CORT and ACTH was tested by radioimmunoassay. RT-PCR was used to test the expression of CRH mRNA of hypothalamus. RESULTS： BCPT（40,80 mg·kg-1 ）improved the reward reaction by increasing sweet water consumption, decreasing the expression of CRH mRNA of hypothalamus and the blood level of CORT and ACTH in chronic stressed rats obviously. CONCLUSION： BCPT exhibited antidepressant-like effect may in part be associated with regulating the hyperacting of the function of hypothalamic-pituitaryadrenal axis （HPAA）.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第10期1162-1165,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省科技厅重点科研项目(№04023048)安徽省教育厅重点科研项目(№2004KJ194ZD)
