摘要
目的:探讨神经元型一氧化氮合酶(nNO S)在创伤性脑损伤(TB I)中的作用。方法:采用落体法大鼠TB I动物模型,观察特异性nNO S抑制剂7-硝基吲唑(7-N I)对TB I早期脑水肿和病理变化的影响。结果:与伤后6 h组相比,7-N I能显著减少脑组织含水量和脑组织中N a+的含量(P值均<0.05),而增加K+的含量(P<0.05),并能改善脑创伤后的病理变化。7-N I的作用可被L-精氨酸逆转,D-精氨酸无效。结论:nNO S来源的一氧化氮对TB I早期起毒性作用,nNO S抑制剂有可能成为治疗继发性脑损伤的新途径。
Objective：To investigate the function of neuronal nitric oxide synthase （nNOS） in traumatric brain injury （TBI）. Methods：Using a rat model of focal cortical contusion according to Feeney,we studied the effect of nNOS inhibitor 7-nitroindazole（7-NI） on early traumatric cerebral edema and pathologicchange in cerebral cortex. Results ：Comparing with 6 hours after TBI group,administration of 7-NI significantly mitigated traumatric cerebral edema,and decreased the content of Na^＋ within brain （P〈0. 05 ,P〈0. 05 ,respectively） ,and increased the content of K^＋ （P〈0.05）. Histological examination showed that cortical contusion was improved treated with 7-NI. The effect of 7-NI was reversed by coadminstration of L-arginine, but not D arginine. Conclusion ： Nitric oxide derived from nNOS may act as a neurotoxic mediator in early traumatric brain injury, nNOS inhibitors may be used to treat secondary brain injury in future.
出处
《中国误诊学杂志》
CAS
2005年第15期2806-2807,共2页
Chinese Journal of Misdiagnostics
