摘要
我们测定了大鼠局部皮肤切除后创面自然愈合过程中,肉芽组织内肿瘤坏死因子(TNF)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量的动态变化,以探讨它们在创伤修复中的变化或相互关系。结果表明:伤后3、5、7天,TNF、SOD 呈Ⅴ型改变,TNF 含量第7天与第5天、SOD 含量第7天与第3天有显著差异(P<0.05),MDA 含量逐渐升高,第7天与第3天比较有显著差异(P<0.05)。相关性检验表明:TNF 与 MDA 各时间点均呈显著正相关。TNF 与 SOD 在伤后3、7天呈显著正相关。TNF 含量<90ng/g 蛋白时创面愈合最佳,SOD 低浓度时创面愈合延缓。提示:在局部创伤修复过程中存在 TNF、MDA、SOD 的变化;内源性低浓度 TNF 及抗氧化能力的提高,对创面修复是有益的。
An experimental model was used to determine the changes in TNF、MDA and SOD in granulation tissues during natural wound healing after skin excision on rats.Our results indicated that the changes in TNF and SOD exhibited a curve of V.The levels of TNF and SOD were lower on day 7 than day 3.The levels of MDA rose gradually,especially on day 7.A positive correlation was shown be- tween TNF and MDA(at days 3、5、7),also between TNF and SOD(at day 3、7).When the concentra- tion of TNF was lower than 90 Pg/mg protein,the process of wound healing was best,while wound healing was hindered when the levels of SOD were low.The results suggest that in the process of wound repair there are influential changes in the contents of TNF,MDA and SOD.Lower levels of TNF and higher levels of SOD are apparently beneficial to wound healing after trauma.
关键词
创伤
修复
肉芽组织
肿瘤坏死因子
丙二醛
Wound healing
Granulation tissue
Tumor necrosis factor
MDA
SOD
