摘要
实验证实了白细胞介素２－绿脓杆菌外毒素融合蛋白ＩＬ２－ＰＥ４０ＲＥＤＬＫ、ＩＬ２－ＰＥ４０ＫＤＥＬ、ＩＬ２－ＰＥ６６4GluＲＥＤＬＫ、ＩＬ２－ＰＥ６６4GluＫＤＥＬ对含高亲和力ＩＬ－２受体靶细胞的杀伤效应是借助于ＩＬ－２与其受体的特异性结合和ＰＥ的杀伤活性介导的，而且同一靶细胞对这些融合蛋白的敏感性有较大的差异，对人外周血ＰＨＡ体外活化的淋巴母细胞的杀伤活性，ＩＬ２－ｐＥ６６4GluＫＤＥＬ要比ＩＬ２－ＰＥ４０ＲＥＤＬＫ强约４０倍，还发现若将ＩＬ２－ＰＥ融合蛋白羧基末端的ＲＥＤＬＫ氨基酸突变为ＫＤＥＬ可增强其杀伤细胞的活性，对ＣｏｎＡ活化的小鼠脾母细胞的杀伤活性，ＩＬ２－ＰＥ４０ＫＤＥＬ要比ＩＬ２－ｐＥ４０ＲＥＤＬＫ强８倍。
We confirmed that the IL 2-PE fusion proteins such as IL2-PE4SREDLK , IL2- PE40KDEL ,IL2-PE66 REDLK and IL2-PE66 KDEL had potent cytotoxicity on the target cells with high affinity IL-2R through the specific binding of IL-2 moiety to IL-2R and the toxicity of the PE moiety. The results showed that the sensitivity of the same target cells to various IL2-PE fusion proteins were different ( for example , the cytotoxicity of IL2-PE66 KDEL on the PHA-activated blasts of human peripheral blood was about 40 times as much as that of IL2-PE40REDLK ) .It was also discovered that the carbonyl terminal REDLK sequence of IL-2-PE fusion proteins substituted by KDEL would enhance their cytotoxicity ( for example , the cytotoxicity of IL2-PE40 KDEL on .the ConA-stimulated murine spleen blasts was about 8 times than the one of IL2-PE40REDLK ) .
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1996年第1期37-40,共4页
Chinese Journal of Microbiology and Immunology
基金
本课题受"863"
国家自然科学基金
全军青年科学基金
广东省青年科学基金资助
关键词
IL-2
绿脓杆菌外毒素
融合蛋白
细胞毒活性
Interleukin-2
Pseudomonas exotoxin
Fusion protein
Cytotoxicity Sitedirected mutagenesis
