摘要
目的:建立了用微波消解技术进行样品前处理,磷钼蓝分光光度法测定食品中磷含量的方法.方法:使用微波消解炉,在食品中加入硝酸密闭消解,消解液用磷钼蓝分光光度法在 660 nm 波长处测定样品中磷的含量.结果:微波消解技术可将样品消解完全,采用磷钼蓝分光光度法测定体系中磷的含量在 0～60 μg 时,磷的含量与吸光度呈线性关系,相关系数为0.9998;用本法测定了国家标准参考物质小麦粉(GBW08503)和贻贝(GBW08571),测定值与标准参考值相吻合,相对标准偏差分别为 0.8% 和 2.2%, 对实际样品的加标平均回收率在 94.9%～109% 之间.结论:该法操作简单、试剂用量少、重现性好,用微波消解技术大大缩短了样品前处理的时间,适于食品中磷元素含量的测定.
Objective：To set up a method for the determination of phosphorous in food samples by ammonium molybdate spectrophotometry after pretreated with the microwave digestion technique. Methods： HNO3 was put in the food sample, which was sealed digested using microwave digestion oven, and the digested solution was anglyzed by ammonium molybdate spectrophotometry at 660 nm wavelength. Results ：The food samples could be digested thoroughly with the microwave digestion technique, and the ammonium molybdate spectrophotometry had good relativity of the linearity （ r = 0. 9998） for phosphorous at the range of 0 - 60 μg. The national standard reference material such as wheat flour（GBW08503） and mussel （GBW08571） was determined by this method, and the results accorded with the reference value, RSD was 0. 8% and 2. 2% respectively. The average recoveries of the spiked samples were 94.9% ~ 109%. Conclusion：This method has simple operation, less reagent use, and good repeatabili- ty. The microwave digestion technique is cut down the periods of sample pretreated greatly, and the method can be applied in the determination of phosphorous in food.
出处
《中国卫生检验杂志》
CAS
2005年第11期1295-1297,共3页
Chinese Journal of Health Laboratory Technology
基金
美国NIH研究基金项目(R01AG19181-01A2)
关键词
微波消解
磷
食品
分光光度法
Microwave digestion
Phosphorus
Food
Spectrophotometry
