摘要
在对落叶松毛虫进行综合治理研究时，从得病幼虫体内分离出一株质型多角体病毒（ＤｓＣＰＶ）。鉴于该病毒在国内未见报道，对其进行了形态、生物测定、组织病理及林间防治试验，结果质型多角体为０．１６μｍ×１．５７μｍ；病毒粒子为１６．０ｎｍ×５８．１ｎｍ；３龄和５龄落叶松毛虫幼虫的ＬＣ５０分别为２．８１×１０４ＰＩＢ／ｍＬ和７．１７×１０４ＰＩＢ／ｍＬ；该病毒感染幼虫中肠７２ｈ后形成多角体，１４４ｈ后形成大量的多角体。用１．１７×１０８ＰＩＢ／ｍＬ浓度的病毒悬液进行林间防治试验，其死亡率达８２％以上，平均达８４．６２％。
strain of cytoplasmic polyhedrosis virus (CPV) was separated from the infected larva during the research of integrated pest management of Dendrolimus superans . The morphology, bioassay, histopathology and field test of this CPV were studied. The size of CPV is 0.16μm×1.57μm and the virion is 16.0nm×58.1nm. The LC50 to the 3rd and 5th instar larva of D.superans were 2.81×10 4 PIB/mL and 7.17×10 4 PIB/mL respectively. The polyhedrosis were formed after midgut of larva were infected for 72 h. A large amount of polyhedrosis were formed after 144 h. The mortality was more than 82% and average mortality was 84.62% when using 1.17×10 8 PIB/mL virus suspension to control the pest in field test.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
1996年第4期26-30,共5页
Journal of Northeast Forestry University
基金
内蒙古自治区"八五"攻关课题
关键词
落叶松毛虫
质型多角体病毒
生物测定
害虫
防治
Dendrolimus superans
Cytoplasmic polyhedrosis virus
Bioassay
Pest control
