摘要
为进一步认识白血病抑制因子（LIF）对人髓母细胞瘤生长的生物学作用，我们使用LIF反义寡核苷酸，在髓母细胞内特异性阻断LIF基因表达并观察它对靶细胞的生物学效应。结果发现，被处理细胞的LIF表达降低至RT／PCR检出的阈值之下，抗LIF的免疫组织化学染色亦呈阴性，同时，这些细胞的生长速度明显减缓。相反，用与反义寡核苷酸互补的正义序列处理的细胞无论在基因表达／产生，还是在生长速度方面，均和正常培养细胞的细胞相似，本研究从而提示了一条以LIF为对象的髓母细胞瘤以及其它LIF生长依赖性肿瘤的基因和免疫治疗途径。
In order to analyse the functional importance of leukemia inhibitory factor (LIF) in the prolliferation of human medulloblastoma cells, antisense LIF oligonucleotides were used to block LIF gene expression in established medulloblastoma cell line Med-3. The effects of this measure on Med-3 cells were then investigated by using different approaches. The results revealed that LIF expression in the cells treated by antisense LIF reduced below the threshold being detectable with RT-CPR and antiLIF irnmunocytochemistry also showed a negative lebelling. Meanwhile, proliferation rate of antisense treated cells decreased distinctively. In contrast, the LlF gene expression as well as proliferation of Med3 cells treated by sense oligonucleotides remained similar as that of the normally cultured cells. The data suggest a LIF oriented gene/immune trial for the therapy of medulloblastomas and other LlF growth-associated tumors of humans
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1996年第3期132-134,共3页
Chinese Journal of Pathology
基金
卫生部优秀青年科技人才基金
辽宁省科委博士启动基金
瑞士国家科学基金
