摘要
目的通过观察拉坦前列素酸对人 Tenon 囊成纤维细胞(human Tenon fibroblast,HTF)Ⅰ型胶原mRNA 表达的影响,研究拉坦前列素对滤过道疤痕化的影响。方法体外培养的 HTF 细胞,分别以不同浓度和不同时间拉坦前列素酸作用后,采用 RT-PCR 方法检测Ⅰ型胶原前胶原α_2亚单位 mRNA 相对表达量的变化。结果拉坦前列素酸作用2d,Ⅰ型胶原 mRNA 10^(-5)M 组下降40.2±8.2%(P<0.01),10^(-6)M 组下降38.4±6.4%(P<0.01),10^(-7)M 组下降29.1±10.5%(P<0.01),10^(-8)M 组下降23.3±10.6%(P<0.01)。10^(-7)M 拉坦前列素酸处理组相对于对照组Ⅰ型胶原 mRNA 第1天下降24.4±9.9%,第2天下降49.0±8.3%,第3天下降94.8±7.1%,随时间延长下调作用增强(P<0.01)。结论拉坦前列素酸可下调 HTF 细胞Ⅰ型胶原α_2亚单位mRNA 的表达。
Purpose To investigate the effect of latanoprost free acid （LP-A） on collagen type Ⅰ mRNA expression in human Tenon fibrohlast （HTF）. Methods Different concentration and different time reaction of LP-A was administrated on cultured HTFs. The expression of mRNA of α2 subunit of procollagen Ⅰ was investigated by RT-PCR. Results After 10^- 9M - 10^-5 M LP-A treating for 2 days, collagen type Ⅰ mRNA expression was down-regulated, 40.2 ± 8.2% （ P 〈 0.01） in 10^-5 Mgroup, 38.4±6.4% （P〈0.01） in 10^-6 Mgroup, 29.1± 10.5% （P〈0.01） in 10^-7M group, and 23.3±10.6% （ P 〈 0.01 ） in 10 ^- 8 M group respeetively. Atier 10^- 7 M LP-A treating for 1 day, collagen Ⅰ mILNA was down- regulated 24.4 ±9.9%, 2days49.0±8.3% , and 3 days94.8±7.1%, inatime-dependent manner （P〈0.01）.Conclusion LP-A is able to down-regulate mRNA of α2 subunit of procollagen Ⅰ in human Tenon fibroblast.
出处
《中国眼耳鼻喉科杂志》
2005年第5期290-292,共3页
Chinese Journal of Ophthalmology and Otorhinolaryngology
