摘要
对42例膀胱移行上皮癌标本分别用PCR-SSCP法检测p53基因外显子5～8的突变及免疫组化法检测突变体p53蛋白。结果发现有15例标本有外显子5～8突变,23例标本有p53基因过度表达蛋白,且基因突变频率随肿瘤分化程度愈差,浸润深度愈深而增加,p53蛋白过度表达率高于p53基因外显子5～8的突变率。结果提示膀胱癌的发生与p53基因突变有关,p53基因突变是膀胱癌晚期的常发事件。
Mutations of exon 5 to 8 of p53 gene were detected in 42 cases of bladder carcinomas and 8 cases of normal controls by PCR-SSCP, and overexpression of p53 protein was found in the same specimens with the immunohistochemical method, p53 gene mutations occurred in 35.7% of patients with the bladder carcinomas and p53 protein was found positve in 54.8% of the same samples. More mutations of p53 gene were detected in high-grade and invasive-type tumors. The ratio of overexpression of p53 protein was higher than that of p53 gene mutations. The results showed that mutations of tumor suppressor p53 were correlated with bladder carcinoma, and the presence of p53 gene mutations indicated poor prognosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1996年第2期85-86,共2页
Chinese Journal of Experimental Surgery
