摘要
通过对PCR芯片工作所需的恒温场进行分析研究,使用半导体致冷片为温度控制部件,设计了一种新型的主动式恒温控制系统。温控系统通过改变半导体致冷片工作电流的极性来瞬时切换系统的升、降温状态,通过改变其工作电压来精确调整系统的工作温度,同时利用半导体致冷片的面加热特性,能够快速建立所需的恒温场,且恒温特性好。与其它PCR芯片温控系统相比,由于系统采用了主动式致冷措施,系统的降温速度很快,约为-17℃/s,较大地缩短了降温过程,改善PCR扩增质量。
Through analyzing the constant temperature field required for proper operation of the PCR chip, a new style active constant temperature control system was devised using a semiconductor cooling device as the temperature control component. The system is capable of instantly switching between temperature rising state and temperature falling state by changing the polarity of working current of the semiconductor cooling plate. It can also precisely regulate the working temperature by changing the working voltage. Meanwhile, through facial heating characteristics of the semiconductor cooling plate, the system can quickly establish required constant temperature field with a good constant temperature attribute. Comparing with other PCR chip based temperature control system, the system has a fast temperature drop speed, approximately - 17℃/s, due to the adopted constant cooling measure, so it can drastically shorten the cooling process and greatly improve the PCR quality.
出处
《仪表技术与传感器》
CSCD
北大核心
2005年第10期32-34,共3页
Instrument Technique and Sensor
关键词
PCR芯片
恒温控制
半导体
PCR Chip
Constant Temperature Control
Semiconductor
