摘要
引入固定化波吉卵囊藻(Oocystis borgei)和微绿球藻(Nannochloris oculata)于凡纳对虾(Litop Penaeus vannamei)养殖环境中,检测水体、对虾胃和后肠中弧菌的数量变化,研究固定化微藻对虾池弧数量动态影响。结果表明:波吉卵囊藻培养液中9d后不能检测出弧菌,微绿球藻培养液中15d后不能检测出弧菌。引入固定化波吉卵囊藻和微绿球藻的褐藻胶藻珠能抑制弧菌的生长,实验组养殖水体、对虾胃和后肠中弧菌的数量都比对照组低;抑制效果是固定化波吉卵囊藻和微绿球藻混合>固定化波吉卵囊藻>固定化微绿球藻;试验后期实验组弧菌的数量明显低于试验前期。试验期间固定化波吉卵囊藻和微绿球藻的生物量分别增加了约10倍和17倍,证明它们的生理活性不会因固定化而受干扰。因此,固定化微藻可应用于虾池微生态调控防病。
There is close relationship between algae and microbes. The structure of bacterial community can be controlled by microalgae in the culture system of shrimp. The physiological capability capabilities of the microalgal cells are increased by the immobilization. After immobilized Oocystis borgei and Nannochloris oculata are artificially introduced into the culture system of Litop Penaeus vannamei ,the numbers of Vibrio are inspected in the water, stomach and intestine posterior of shrimp to study the influence of immobilized microalgae over the quantity dynamics of Vibrio. The result shows that there is not detectable Vibrio in both the culture systems with Oocystis borgei, Nannochloris oculata after 9 days, 15 days culture in this experiment respectively. There is not Vibrio in the culture system with microalga in the late period of culture. Introducing the algal alginate-beads of Oocystis borgei and Nannochloris oculata into cultural environment of shrimp can inhibit growth of Vibrio. The numbers of Vibrio in the water, stomach and intestine posterior of shrimp in the culture system with Oocystis borgei, Nannochloris oculata. Oocystis borgei mixing with Immobilized Nannochloris oculata are depressed 69.92% , 63.67% , 72.07% ; 71.76%, 70.79% , 79.30% and 80.22% ,79.12% ,81.78% respectively in contrast to the control group. The inhibitory effect of immobilized Oocystis borgei mixing with immobilized Nannochloris oculata is brtter than immobilized Oocystis borgei and immobilized Oocystis borgei is better than immobilized Nannochloris oculata. The Vibrio numbers of the experiment group in the late period of the experiment are less than the early. The microalgas cell numbers in the algal beads begin to increase at second day of this experiment. The color of the algal alginate-beads is bottle green in the late period of the experiment. The cell numbers of immobilized Oocystis borgei and Nannochloris oculata in the per bead increased from 0.33×10^7 to 3.17×10^7 cells and 1.50×10^7 to 25.47×10^7 cells respec-ively and the biomass is increased about 10 and 17 times respectively in 21days of culture in this experiment. This proved that the physiological capabilities of the immobilized microalgal cells are not interrupted by the immobilization. Thus,the immobilized microalgae can be used to micro-ecology control to prevent shrimp disease in the shrimp pond.
出处
《水生生物学报》
CAS
CSCD
北大核心
2005年第6期684-688,共5页
Acta Hydrobiologica Sinica
基金
广东省科技攻关项目(编号:C20823)资助
关键词
固定化微藻
褐藻胶藻珠
细菌
微生态调控
Immobilized microalgae
Algal alginate-bead
Bacteria
Micro-ecology control
