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粘着斑激酶在TF/Ⅶa介导的乳腺癌细胞粘附、迁移中的作用 被引量：1

The role of focal adhesion kinase in the breast carcinoma 's adhesion and migration mediated by the coagulation factor Ⅶa/tissue factor
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摘要 目的研究粘着斑激酶(FAK)磷酸化在凝血因子Ⅶa与组织因子(TF)结合引起的乳腺癌细胞粘附、迁移中的作用,探讨TF促肿瘤转移的机制。方法用比色法测定凝血因子Ⅶa与高表达TF的乳腺癌MDA-MB231细胞的粘附数目;采用改良Bodyen小室检测肿瘤细胞迁移;免疫沉淀和Western blot方法检测FAK及其磷酸化。结果凝血因子Ⅶa与TF结合引起FAK磷酸化,参与肿瘤细胞迁移运动。结论FAK是TF介导的乳腺癌细胞粘附、迁移中的重要信号分子。 Objective To study the role of focal adhesion kinase in the breast carcinoma cell adhesion and migration mediated by coagulation factor Ⅶ a combined with tissue factor to explore the mechanism of tissue factor promoting metastasis. Methods The count of adherent cells were detected by using colorimetric assay, cell migration was detected with modified Bodyen chamber, and the FAK and the tyrosine phosphorylation of FAK were detected by immunoprecipitation and Western blot. Results Factor Ⅶa combinaed with tissue factor induced the tyrosine phosphorylation of FAK, which took part in the cancer cell＇s migration. Conclusion FAK is an important message of the breast carcinoma＇s adhesion and migration mediated by TF.
作者 郭艳丽 魏文宁 宋善俊
机构地区 华中科技大学同济医学院附属协和医院血研所
出处 《西安交通大学学报（医学版）》 CAS CSCD 北大核心 2005年第5期467-469,共3页 Journal of Xi’an Jiaotong University（Medical Sciences）
关键词 粘着斑激酶 组织因子 粘附 迁移 focal adhesion kinase tissue factor adhesion migration
分类号 R730 [医药卫生—肿瘤]
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参考文献7

  • 1方峻,宋善俊.活化的血液凝固因子Ⅶa和组织因子复合物介导信号传导与肿瘤生长、浸润及转移[J].中华血液学杂志,2002,23(5):279-280. 被引量：15
  • 2方峻,魏文宁,夏凌辉,宋善俊.TF-F Ⅶa复合物影响人卵巢癌细胞尿激酶型纤溶酶原激活物受体mRNA表达的研究[J].中华血液学杂志,2004,25(3):143-146. 被引量：19
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二级参考文献11

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共引文献29

同被引文献12

  • 1LLOVET JM,BEAUGRAND M. Hepatocellular carcinoma:present status and future prospects[J].Journal of Hepatology,2003,(Suppl):136-149.
  • 2TANG ZY,YE SL,LIU YK. A decade's studies on metastasis of hepatocellular carcinoma[J].Journal of Cancer Research and Clinical Oncology,2004,(04):187-196.doi:10.1007/s00432-003-0511-1.
  • 3FRAU M,BIASI F,FEOF. Prognostic markers and putative therapeutic targets for hcpatocellular carcinoma[J].Molecular Aspects of Medicine,2010,(02):179-193.
  • 4LI SF,HUA ZC. FAK expression:regulation and therapeutic potential[J].Advances in Cancer Research,2008,(01):45-61.
  • 5SCHWOCK J,DHANI N,HEDLEY DW. Targeting focal adhesion kinase signaling in tumor growth and metastasis[J].EXPERT OPINION ON THERAPEUTIC TARGETS,2010,(01):77-94.doi:10.1517/14728220903460340.
  • 6PYLAYEVA Y,GILLEN KM,GERALD W. Ras-and PI3K-dependent breast tumorigenesis in mice and humans requires focal adhesion kinase signaling[J].Journal of Clinical Investigation,2009,(02):252-266.
  • 7BATEMAN NW,SUN M,HOOD BL. Defining central themes in breast cancer biology by differential proteomics:conserved regulation of cell spreading and focal adhesion kinase[J].Journal of Proteome Research,2010,(10):5311-5324.
  • 8WENDT MK,SMITH JA,SCHIEMANN WP. Transforming growth factor-beta-induced epithelial-mesenchymal transition facilitates epidermal growth factor-dependent breast cancer progression[J].Oncogene,2010,(49):6485-6498.
  • 9MCLEAN GW,CARRAGHER NO,AVIZIENYTE E. The role of focal-adhesion kinase in cancer.A new therapeutic opportunity[J].Nature Reviews Cancer,2005,(07):505-515.
  • 10LUO M,GUAN JL. Focal adhesion kinase:A prominent determinant in breast cancer initiation,progression and metastasis[J].Cancer Letters,2010,(02):127-139.

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西安交通大学学报（医学版）
2005年 第5期

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