摘要
用鼠抗人ＣＤ３、ＣＤ４、ＣＤ８单克隆抗体（ＭＣＡｂ）识别精液中的淋巴细胞，用羊抗氧荧光抗体（ＦＩＴＣ）作为第２抗体染色镜检，细胞膜呈草绿色荧光为阳性。结果表明，生育男性与精索静脉曲张及附睾结核的男性精液中Ｔ淋巴细胞检出率差异显著（Ｐ＜０．０１）。
This study was designed to detect the T lymphocytes in semen with anti-human CD3, CD4, CDS monoclonal antibodies, which stained by the second antibody sheep anti-mouse-FITC. The result showed that the percentage of lymphocytes were signficantly different in semen between fertile group and palpable varicocele or tuberculosis of epididymis (P<1. l1).
