摘要
目的:研究胎盘促肾上腺皮质激素释放激素(corticotrophin releasing hormone,CRH)对雌激素合成的促进作用,从而探讨CRH启动分娩的机制。方法:分离正常人胎盘滋养层细胞并培养72h,分别用不同浓度CRH和CRH受体拮抗剂αHelicalCRH941处理24h,应用放射免疫方法测定细胞上清中雌二醇含量,提取细胞总RNA,用Northern印迹杂交检测雌激素合成关键酶———芳香化酶(P450aromatase,P450arom)mRNA表达量的变化。结果:CRH明显促进胎盘细胞合成雌二醇,并显著增加P450arommRNA的表达;αHelicalCRH941不仅能阻断CRH对雌二醇合成和对P450arommRNA表达的促进作用,还可抑制雌二醇的合成和P450arommRNA的表达(P<0.01)。结论:胎盘CRH促进雌二醇的合成,这种作用与其促进芳香化酶的表达有关。CRH促进激活子宫的激素———雌二醇的合成可能是其启动分娩的机制之一。
Objective： To study the effect of corticotrophin-releasing hormone（CRH） on estrogen production in human trophoblasts and its mechanisms. Methods： Cytotrophoblasts were isolated from term human placentas and cultured for 72 h. The cultured cells were then treated with various concentrations of CRH and CRH receptor antagonist,α-Helical CRH9-41 for 24 h; estradiol was measured by radioimmunoassay in the culture media. Expression of aromatase（P450arom） ,the key enzyme for estrogen synthesis,was analyzed by Northern blot. Results： CRH significantly stimulated estradiol production and the expression of P450arom mRNA in placental cells. It was found that α-Helical CRH941 blocked the effect of CRH and inhibited estradiol production and P450arom mRNA expression（P〈0.01）. Conclusion： Placental CRH increases estrogen synthesis, which may be attributable to the promoting effect of CRH on P450 expression. Activation of estradiol by CRH may contribute to the process of parturition in humans.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第10期1128-1130,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30170982)
