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溶脲脲原体感染者中输血传播病毒DNA的检测

DETECTION OF TRANSFUSION TRANSMITTED VIRUS IN UREAPLASMA UREALYTICUM PATIENTS.
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摘要 目的:调查输血传播病毒(TTV)在溶脲脲原体感染者中的感染状况,探讨TTV的传播途径。方法:在TTV ORF1保守区设计引物,建立套式聚合酶链反应(NPCR),检测52例溶脲脲原体感染者和34例普通体检人群分泌物中TTV-DNA。结果:在52例溶脲脲原体感染者中检出19例TTV-DNA阳性,检出率为36.5%,而普通体检人群TTV-DNA阳性率为2.9%,两组差异有统计学意义(χ2=13.0 P<0.01)。结论:溶脲脲原体感染者是TTV感染的高危人群,性接触是TTV传播的重要途径之一。 Objective: To investigate the infection status of transfusion transmitted virus (TTV) in ureaplasma urealyticum patients. Methods: A nested polymerase chain reaction (NPCR) assay with primers from ORF1 of TTV genome was used to detect TTV DNA in the secretion samples of 52 UU patients and 34 general somato-scopic population. Results: The results showed that 19 of the 52 (36.5%) UU patients and 1 of 34 (2.9%) general somato scopic population were positive for TTV DNA (P〈0.01). Conclusion: The finding suggests that the UU patients are also the high risk population for TTV infection, and sexual contact is one of important routes for the transmission of TTV.
出处 《现代预防医学》 CAS 北大核心 2005年第11期1483-1484,共2页 Modern Preventive Medicine
关键词 输血传播病毒 溶脲脲原体 聚合酶链式反应 分泌物 Transfusion transmitted virus (TTV) Ureaplasma urealytieum (UU) Nested polymerase chain reaction (NPCR) Secretion
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参考文献12

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