摘要
目的:建立准确、快速的风疹病毒内感染实验诊断方法。方法:将疫苗株病毒稀释后接种Vero细胞,收集不同时段培养上清液和细胞,RT-PCR法检测风疹病毒RNA(培养RT-PCR法)。用直接RT-PCR法和培养RT-PCR检测可疑风疹病毒感染的胎儿组织和羊水标本。结果:直接RT-PCR法可检测模拟羊水标本中15 TCID50/ml风疹病毒RNA。培养RT-PCR法在10 TCID50的病毒感染后6h的V细胞和感染后24h的培养上清液中检出风疹病毒RNA。直接RT-PCR法和培养RT-PCR法检测4例足月产胎儿羊水均阴性;1例引产胎儿羊水直接RT-PCR法阴性,培养RT-PCR法在接种细胞后24h检出风疹病毒RNA。结论:培养RT-PCR法是一个快速、特异、灵敏的实验室诊断风疹病毒宫内感染的方法。
Objective： To develope a reliable and rapid method for prenatal diagnosis of intrauterine rubella virus infection. Methods： Vero cells were infected by rubella vaccine virus, and the supernatant and cells were collected at given time for RNA extraction and detected by RT- PCR （culture-RT-PCR）. Tissues and amniotic fluid of high suspected fetus of intrauterine rubella virus infection were detected by direct RT-PCR and culture-RT-PCR. Results： The direct RT- PCR gained positive results when the titer of model samples was 15 TCID50/ml and above. The culture-RT-PCR detected the virus RNA in 5 h＇ cells and 24 h＇ supernatant post infection of vero cells infected by 10 TCID50 of rubella vaccine virus. The culture-RT-PCR had the positive result at 6 h＇ Vero cells and 24 h＇ supernatant post infection of 10 TCID50 and above. Employing direct RT-PCR and cuture RT-PCR, 4 term birth＇ amniotic fluid were all negative of rubella virus RNA. One induced amniotic fluid had a positive result by culture-FT-PCR at 24 h＇ cells post inoculation, while the direct RT-PCR was negative. Conclusion： Culture-RT-PCR is a rapid, specific and sensitive method to diagnose the intrauterine rubella virus infection.
出处
《现代预防医学》
CAS
北大核心
2005年第11期1438-1440,共3页
Modern Preventive Medicine
基金
温州市计委重点攻关项目(1998594)
