摘要
本研究利用反转录聚合酶链式反应(RT-PCR)观察了将氯胺酮给予生后早期大鼠后,海马组织中NMDA受体亚型mR-NA的表达变化。实验用生后7d的SD大鼠40只,随机分为2大组:给药后即刻组(PND7组)和给药后3周组(PND28组)。PND7组为腹腔注射不同剂量氯胺酮后24h内处死,PND28组用相同给药方法给药并在相同环境中饲养3周(即至生后28d)后处死。RT-PCR结果显示在PND7组,腹腔注射氯胺酮引起海马组织内NR2A,NR2B和NR2C亚型mRNA的表达上调,NR1受体亚型mRNA的表达无明显变化。在PND28组,氯胺酮注射引起NR1和NR2A亚型mRNA的表达水平增高,而NR2B和NR2C亚型mRNA的表达没有变化。氯胺酮的给药量和给药方式对上述受体亚型的表达变化没有明显影响。各受体亚型在不同发育阶段的海马组织中的表达水平也有一定的差异。本研究的结果提示氯胺酮对发育阶段大鼠脑内NMDA受体的表达具有上调作用,从而对大鼠神经系统的发育可能产生影响。
In the present study, reverse transeription -polymerase chain reaction （RT-PCR） technique was used to detect the changes in the expressinn of NMDA receptor subunit mRNAs in the hippocampus of neonatla rats after ketamine treatment. Rats （ n =40） at postnatal day 7 （ PND7 ） were used and divided into two groups; PND7 group in which rats were killed 24 h after ketamine treatment with different doses, and PND28 group in which rats were killed 3 weeks after ketamine treatment in similar way. RT-PCR results showed that in the PND7 group, intraperitoneal injection of ketamine resuhed in an increased expression of NR2A, NR2B and NR2C subunit mRNAs in the hippocampus. No such change was observed in the expression of NR1 subunit mRNA. In PND28 group, ketamine administration increased the expression of NRI and NR2A suhunit mRNAs in the hippocampus, while the expression of NR2B and NR2C subunit mRNAs remained unchanged. The dose and administration mannner of ketamine seemed no obvious effects on the expression of NMDA subuntis. Different expression level of above mentioned NMDA subunit mRNAs were observed in the hippocampus at different developmeenal stages. The present results suggested that ketamine could increase the expression of different NMDA receptor subunits in the developmental rat brain anti may influence the development of the rat nervous system.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2005年第5期451-455,共5页
Chinese Journal of Neuroanatomy
基金
陕西省自然科学基金(2003K4G10)资助项目
