摘要
目的:了解长沙地区淋病奈瑟菌对抗生素的敏感性以及耐青霉素和耐四环素性质粒的流行情况。方法:采用纸片扩散法检测80株淋病奈瑟菌对7种抗生素的敏感性;碱裂解法分析产青霉素酶的淋病奈瑟菌(PPNG)株及耐四环素的淋病奈瑟菌(TRNG)株的质粒图谱;PCR扩增tetM基因片段,并经测序分析其基因型。结果:在测定的7种抗生素中,耐药性最高的为环丙沙星,耐药率为86.25%,敏感性最高的为大观霉素,为100%;PPNG和TRNG的检出率分别为50.0%和27.5%;40株PPNG株质粒电泳后发现除1株为非洲型耐青霉素质粒外,其余均为亚洲型耐青霉素质粒;22株TRNG株的PCR产物电泳条带均在约443bp处,取典型株经DNA测序分析与荷兰型tetM基因具有96%～98%的同源性。结论:质粒介导的淋病奈瑟菌对青霉素和四环素的耐药已普遍存在,本次研究中介导的耐青霉素和耐四环素质粒分别以亚洲型和荷兰型为主。
Objective： To investigate the antibiotic resistance and the epidemic situation of penicillin and tetracycline resistance plasmids of Neisseria gonorrhoeae in Changsha area. Methods： Susceptibility to 7 antibiotic agents was tested by the disc diffusion method in 80 isolates,and plasmid DNA was obtained by alkaline lysis method in PPNG and TRNG strains, TerM genetypes were detected by PCR amplification and sequenced analysis. Results： Of all the 7 antibiotic agents, ciprofloxacin had a highest resistance and the percentages of resistance was 86.25%,at the same time, spectinomycin had a highest susceptibility and the percentage of susceptibility was 100.00%. PPNG and TRNG were 50.00% and 27.50% respectively. Forty PPNG strains tested carried the 7.2 kb （Asian type） plasmid except one carrying the 5.3 kb （African type） plasmid. All the PCR productions of 22 TRNG strains showed the bands about 443 bp. Classical strains were directly sequenced and its nucleotide sequences were 96%-98% similar to Dutch-type terM gene. Conclusions： plasmid-mediated resistance to penicillin and tetracycline is common, and the Asian type PPNG plasmid and the Dutch type TRNG plasmid are in the absolute majority of resistance strains in this study.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2005年第10期659-661,共3页
Journal of Clinical Dermatology
关键词
淋病奈瑟菌
抗生素
敏感性
质粒
耐药性
长沙市
Neisseria gonorrhoeae
antibiotic susceptibility
plasmids,resistance
Changsha City
