摘要
目的探讨羊膜细胞培养液治疗兔眼重度碱烧伤的机制,为临床应用提供理论依据。方法采用中国大白兔72只,随机分成对照组(A组)24只,0.1%地塞米松治疗组(B组)24只,羊膜上皮细胞培养液治疗组(C组)24只。各组动物采用单眼建立角膜碱烧伤模型并分别以0.1%地塞米松液和羊膜细胞提取液对B、C组动物伤眼进行治疗。于伤后1、3、7、14 d活杀动物。每个时段各组分别活杀6只兔,取眼房水检测TNF-α、IL-1的含量与活性。结果各时段B、C治疗组与对照组相比TNF-α、IL-1的活性和含量明显降低(P<0.01)。结论抑制TNF-α和IL-1的生物合成和释放可能是羊膜细胞培养液治疗角膜碱烧伤的机制之一。
Objective To investigate the therapeutic mechanism of extract of human amniotic cells in treating rabbit corneal alkali burn and to provide the rational evidence for its clinical application. Methods The rabbit model of corneal alkali burn was established by 0.5moL/L sodium hydroxide to one eye of each rabbit. All 72 rabbit models were randomly divided into 3 groups as control group,0.1% Dex group and human amniotic cells extract group. TNF-α, IL-1 were detected in aqueous humor at day 1,3,7, 14 after burn. Results As compared with control group, the expression of TNF-α, IL-1 were obviously lower in the groups treated with 0.1% Dex or human amniotic cells extract（P 〈0.01 ）. There was no statistical difference in TNF-α, IL-1 levels between 0.1% Dex group and human amniotic cells extract group. Conclusion Inhabiting the biosynthesis and release of TNF-α, IL-1 is the possible mechanism of human amniotic cells extract in managing the rabbit corneal alkali burn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第17期1776-1777,共2页
Journal of Third Military Medical University
关键词
羊膜
角膜
碱烧伤
肿瘤坏死因子
白细胞介素-1
amniotic epithelial cell
cornea
alkali burn
tumor necrosis factor-α
inerleukin-1
