摘要
目的探讨异丙酚持续恒速输注在大鼠无肝期前后血药浓度变化规律、肝外代谢、肺脏对异丙酚肝外代谢的作用。方法通过大鼠左颈深静脉持续恒速输注异丙酚,于分离肝门时、肝门阻断即刻、肝门阻断15、30、60 m in等时间点,同时从右侧颈深动、静脉分别采血(100μl),采用高效液相色谱荧光法(HPLC)检测异丙酚血药浓度。结果颈深静脉或颈深动脉异丙酚血药浓度各时相点间比较,肝门阻断15、30、60 m in时的血药浓度比阻断前和阻断即刻明显升高(P<0.01),但3组间没有显著性差异(P>0.05);颈深静脉与颈深动脉异丙酚血药浓度比较,任何时刻均无显著性差异(P>0.05)。结论肝脏是异丙酚主要代谢器官,异丙酚存在肝外代谢,肺脏不是异丙酚肝外代谢的主要器官。
Objective To observe the changes of propofol blood concentrations in rats during anhepatic phase by continuous constant-speed infusion of propofol and to investigate the extrahepatic metabolism of propofol and the effects of lungs on the extrahepatic metabolism of propofol. Methods When the continuous constant-speed transfusion of propofol was carrying out through the left deep cervical vein, the blood samples of 100 txl were withdrawn simultaneously from the right deep cervical artery and vein at the flowing time points： 5 min before the dissociation of hepatic portal, immediate devascularization of the hepatic portal, 15 min, 30 min and 60 min after devascularization of the hepatic portal. The propofol blood concentrations were analyzed by HPLC. Results At 15 min, 30 rain and 60 min after the devascularization of the hepatic portal, the propofol blood concentrations in the right deep cervical artery and vein were significantly higher than that at 5 min before the dissociation of hepatic portal and immediate devascularization of the hepatic portal （ P 〈 0.01 ）. The propofol blood concentrations were of no significant difference at 15, 30 and 60 min after the devascularization of the hepatic portal. The blood propofol concentrations in the deep cervical artery and vein were of no significant differences at any time points. Conclusion The liver is the main organ for propofol metabolism. Extrahepatic metabolism of propofol exists but the lung is not the main organ for propofol extrahepatic metabolism.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第17期1766-1768,共3页
Journal of Third Military Medical University
基金
第三军医大学青年科研基金资助项目(2004)~~
关键词
异丙酚
无肝期
肝外代谢
propofol
anhepatic phase
extrahepatic metabolism
