摘要
目的通过观察大鼠肺内eotaxin表达的变化,探讨氨茶碱治疗哮喘的可能机制。方法以OVA致敏并激发大鼠哮喘,以不同剂量的氨茶碱治疗,分别通过免疫组化和RT-PCR检测肺内eotaxin蛋白及mRNA的表达。结果大、中、小剂量的氨茶碱均能明显抑制哮喘大鼠肺内eotaxin的表达(与未治疗组相比P<0.05)。结论氨茶碱可通过抑制eotaxin的表达减轻哮喘炎症。
Objective To investigate the eotaxin expression in rat lungs and discuss the possible mechanism of therapeutic effect of aminophylline on asthma patients. Methods A total of 50 rats were randomized into asthma group （n = 10）, aminophylline group （n = 30） and control group （n = 10）. The asthma group and aminophylline group were sensitized and challenged with OVA, while the control group with 20% aluminium hydroxide gel. The aminophylline group was respectively administrated intraperitoneally 100 mg/ml, 25 mg/ml, 5 mg/ml aminophylline at dose of 1 ml half an hour before the stimulation every day. In 24 h after the last stimulation, the rats were all sacrificed and eotaxin protein and mRNA were investigated with immunohistochemistry and RT-PCR techniques respectively. All data were analyzed with Student＇s t test. Results Aminophylline of different doses could inhibit eotaxin expression significantly as compared with the other two groups （ P 〈 0. 05 ）. Conclusion Aminophylline can ameliorate asthmatic inflammation by inhibiting eotaxin expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第17期1763-1765,共3页
Journal of Third Military Medical University
