摘要
目的探讨大肠癌组织中胸腺素β4基因的表达及其临床意义。方法采用逆转录聚合酶链反应(RTPCR)方法,检测41例大肠癌患者肿瘤组织及正常黏膜组织中胸腺素β4基因mRNA的表达。结果半定量RTPCR显示所有癌组织中均有胸腺素β4mRNA表达,但在部分正常黏膜组织中表达缺失。癌组织平均表达量为0.65±0.21,正常黏膜组织为0.42±0.19(P<0.01)。其表达与淋巴结转移和临床分期有关,在有淋巴结转移组中胸腺素β4mRNA表达(0.82±0.23)明显高于无淋巴结转移组(0.53±0.19)(P<0.05);C+D期组中胸腺素β4mRNA表达(0.82±0.22)明显高于A+B期组(0.51±0.18)(P<0.05),与肿瘤大小、分化程度、患者性别、年龄、肿瘤部位无关。结论大肠癌组织中胸腺素β4的表达与肿瘤的转移播散相关,胸腺素β4可作为判断大肠癌转移播散和临床分期的参考指标。
Objective To study the expression of thymosin 134 in colorectal cancer and its clinical significance. Methods Forty-one cases of human colorectal cancer specimens was collected from the patients who received operation for colorectal cancer from 12/2003 to 11/2004. All of the specimens were determined for mRNA expression of thymosin β4 by using RT-PCR technique. Results Thymosin β4 mRNA was high-expressed in colorectal cancer specimens although they were also expressed in normal colorectal specimen. The results of semi-quantitative PT-PCR anatysis showed the expression of thymosin β4 mRNA was 0. 65 ± 0. 21 and 0. 42 ± 0. 19 in cancer and normal tissues, respectively, P〈0. 01. The relative values of mRNA in lesions with metastatic lymph nodes （0. 82 ± 0. 23） was higher than those of non-metastatic lymph nodes （0. 53 ± 0. 19） （P〈0. 05） ; expression of thymosin β4 in Duke C or Duke D stage （0. 82 ± 0. 22） was significantly higher than that in Duke A or Duke B stage （0. 51 ± 0. 18）. There was a close relation between thymosin β4 mRNA expression and several clinicopathological factors including lymph node metastasis, and TNM stage. Conclusion Thymosin β4 gene expression in colorectal cancer may serve as a parameter for judging tumor biological characteristic and clinical significance.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2005年第9期545-548,共4页
Cancer Research on Prevention and Treatment
