摘要
目的:探讨转染癌胚抗原基因pcDNA3-hCEA的人树突状细胞(DC)对胃癌细胞MGC803裸鼠移植瘤生长的影响.方法:采用细胞因子rhIL-4和rhGM-CSF诱导培养法制备人外周血DC,并用L ipofectine向人DC转染pcDNA3-hCEA.RT-PCR检测转染人CEA真核表达质粒的表达.使用DC(pcDNA3-hCEA)疫苗体外诱导人T淋巴细胞靶向性杀伤反应,并在体内实验中观察DC(pcDNA3-hCEA)对MGC803在裸鼠上致瘤性的影响.结果:针对特定引物DC(pcDNA3-hCEA)在588 bp处有CEA片段的表达;DC(pcDNA3-hCEA)能够诱导的人T淋巴细胞靶向性杀伤活性(%),在效靶比分别为10∶1,20∶1,40∶1时的结果为19.4±3.8,24.7±3.7,38.1±5.4;DC(pcDNA3-hCEA)能显著抑制MGC803的生长,其肿瘤生成日期较对照组延长(中位数10 dvs6 d,n=5),瘤体生长速度较对照组缓慢,d 23瘤体体积明显小于对照组[(0.24±0.10)cm3vs(0.65±0.17)cm3,(1.36±0.42)cm3,n=5,P<0.05].结论:转染pcDNA3-hCEA的人DC能有效抑制MGC803在裸鼠体内的生长.
AIM： To investigate the inhibition of dendritic cells （DCs） （pcDNA3-hCEA） inoculation on MGC803 in nude mice. METHODS： DCs were generated from human fresh peripheral blood in the presence of rhGM-CSF and rhlL-4 and DC vaccine was prepared by transfection with pcDNA3-hCEA using lipofectine. CEA mRNA expressing in DCs （ pcDNA3-hCEA ） was confirmed by RT-PCR and specific cytotoxic T lymphocytes （CTL） was induced in vitro. The inhibition of the DC （pcDNA3-hCEA） inoculating on MGC803 in nude mice was observed. RESULTS： CEA mRNA of DCs （pcDNA3-hCEA） could be detected at 588 bp with a pair of specific primers by RT-PCR and the specific CTL （%） at effect-target rate 10：1,20：1,40：1 were respectively 19.4±3.8, 24.7±3.7, 38.1±5.4. DCs （pcDNA3-hCEA） effectively inhibited the growth of MGC803. The median of tumor genesis time in the experiment group was longer than that of control （ 10 d vs6 d, n=5） and the growth speed of tumor inoculating on DCs （pcDNA3-hCEA） in nude mice was slower than that in control group. The size of tumor inoculating on DCs （pcDNA3-hCEA） in nude mice was less than that in control group E （0.24±0.10） cm^3 vs （ 1. 36 ± 0.42 ） cm^3 , n = 5, P 〈 0.05 1. CONCLUSION： The human dendritic cells transfected with pcDNA3-hCEA can inhibit MGC803 cells in nude mice.
出处
《第四军医大学学报》
CAS
北大核心
2005年第19期1802-1804,共3页
Journal of the Fourth Military Medical University
基金
河南省重点科技攻关项目(001170209)
关键词
树突状细胞
癌胚抗原
脂质体
转染
dendritic cell
carcinoembryonic antigen
plasimd
transfection
