摘要
目的建立一种在光学显微镜下识别巨核细胞快速而特异的染色方法,为血液病的诊断和鉴别诊断提供新方法和新思路。方法骨髓或血涂片上滴加10g/L碱性品蓝乙醇染液染5min,再于碱性品蓝乙醇染液上滴加等量碱性品蓝HEPES染液,充分混匀染10min,水洗晾干后镜检。同时与流式细胞技术及细胞化学染色技术对比观察。结果正常和血液病患者的骨髓或血涂片经碱性品蓝双染色后,原巨核细胞、幼巨核细胞、巨核细胞的核亮蓝色,胞质紫红色,血小板染成紫红色,小巨核细胞的核亮蓝色,胞质紫红色。采用同样的方法染色,其他血细胞(如粒细胞系、单核细胞系、淋巴细胞系、浆细胞及幼红细胞等)均无此染色特征。通过与其他免疫组织化学染色方法比较及用流式细胞仪法鉴定,证明碱性品蓝双染色法对巨核细胞染色是可靠而特异的。结论国产纺织染料碱性品蓝作为一种在光学显微镜下识别巨核细胞的染料,具有特异性高,染色方法简便快速等优点。采用碱性品蓝双染色染巨核细胞在血液病诊断和鉴别诊断上具有实用价值。且染料便宜易得,易于在临床推广应用。
Objective To establish a rapid and specific method for identifying megkaryocytes under the light microscope and to provide a new method and new idea for diagnosis and differential diagnosis of blood diseases. Methods Unfixed smears of blood or born marrow were exposed firstly to an alcoholic solution of Basic Royol Blue for 5 rain. Next, an equal amount neutral HEPES buffered solution of the same dye was added to the first stain solution. The two solutions were mixed by adding the second stain. After 10 rain, the smears were washed for 2 rain with flooding water and drained dry. Meanwhile, the stain was compared with traditional histochemistic stains and flow cytophotometer. Results Megakaryocytes showed purple red cytoplasm and bright blue nuclei. Platelet displayed purple red. Other blood cells （such as granulocytes, monocytes, lymphocytes, plasmacytes and ucleated erythrocytes ） had no such stairming character. Compared with traditional histochemistic stains and flow cytophotometer, the stain of two steps by using Basic Royole Blue for megkaroblasts, promegkaryocytes and megkarocytes was credible and distinctive. Conclusion Basic Royol Blue for megkaryocytes stain was worth to contribute to the diagnosis and differential diagnosis of blood diseases. This technique can be used in clinical laboratory.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第9期915-918,共4页
Chinese Journal of Laboratory Medicine
