摘要
目的:研究甘草次酸钠(SG)对乳鼠心肌细胞的作用。方法:体外培养乳鼠心肌细胞,用放射免疫法和荧光法分别测定cAMP和[Ca^(2+)]_i。结果:SG 0.4mmol·L^(-1)于5,10和15min使心肌细胞搏动频率由73±9min^(-1)分别降至62±5,59±7和56±6min^(-1)。SG0.1和0.2mmol·L^(-1)分别在15和10,15min呈现以上相似的结果。SG0.2和0.4mmol·L^(-1)与心肌细胞37℃共孵10,15min,cAMP浓度和[Ca^(2+)]_i均被降低。SG 0.2mmol·L^(-1)处理组的cAMP含量低于对照组(1.09±0.18,1.12±0.35pmol per vial,P<0.05);[Ca^(2+)]_i的变化也类似(30±4nmol·L^(-1),P<0.01,28±6nmol·L^(-1)P<0.05)。SG 0.1和0.2mmol·L^(-1)提高心肌细胞悬液氧分压变化率(87％±5％,75％±4％,P<0.01),但SG0.4mmol·L^(-1)降低氧分压变化率(31％±2％,P<0.01)。结论:SG可保护心肌或治疗缺血性心脏病。
AIM: To investigate the effect of sodium glycyrrhetinate (SG) on neonatal rat myocardial cells. METHODS: The neonatal rat myocardial cells were cultured in vitro. Radioimmunoassay and fluorimetry were used to determine cAMP and [Ca2±], respectively. RESULTS: The beating
mmol·L-1 at5, 10, and 15 min, from 73±9 min-1 to 62 ±5, 59 ±7, and 56 ±6 min-1, respectively. SG 0. 1 and 0.2 mmol·L-1 showed above similar results at 15 min and 10, 15 min, respectively. When the myocardial cells were incubated with SG 0.2 and 0.4 mmol·L-1 at 37 ℃ for 10 and 15 min, the concentration of cAMP and [ Ca2+ ] were reduced. cAMP contents in SG 0. 2 mmol · L-1 treated group at 10 and 15 min were lower than control group (1. 09 ± 0. 18 vs 1. 65 ± 0.48 pmol per vial, P<0.05; 1.12 ±0.35 vs 1.72 ±0.49 pmol per vial, P<0.01), and so was [Ca2+] (30 ±4 nmol·L-1 vs 41 ±6 nmol·L-1, P<0.05 and 28±6 nmol·L-1 vs 38 ± 7 nmol·L-1, P<0.01). SG 0. 1 and 0. 2 nmol · L-1 increased the pO2 change rate (87 % ±5 %, 75 % ±4 % vs 54 % ±3 %, P < 0. 01) in suspension fluid of myo-cardial cells, but SG 0. 4 mmol·L-1 decreased it (31% ± 2 % vs 54 % ± 3 %, P < 0. 01 ). CONCLUSION: SG protects myocardium or treats ischemic cardiac disease.
出处
《中国药理学报》
CSCD
1996年第4期331-333,共3页
Acta Pharmacologica Sinica
关键词
甘草次酸
心肌
腺苷环磷酸
钙
氧
细胞培养
甘草
glycyrrhetinate
myocardium
cultured cells
cyclic AMP
calcium
oxygen
