摘要
沃尔巴克氏体(Wolbachia)是广泛分布于节肢动物体内的一类共生菌,它们参与调控其寄主多种生殖活动的机制。本研究利用PCR方法对wsp基因的特异性扩增和测序,证实了Wolbachia在金小蜂体内的共生。并利用所测序列和其他已发表的序列建立系统树,为研究寄主与拟寄生物间的水平传播机制奠定了基础。
Wolbachia is a kind of symbioant bacteria commonly habiting in arthropod bodies, these bacteria participate in manipulating various reproducing mechanisms of their hosts. In this study, the presence of Wolbachia in Pteromalus venustus was detected based on amplification and sequencing the fragments of the wsp gene by PCR. Kinship relative tree was drawn by aligning the amplified fragment sequence with the published sequences, and the study provided the basis for further researching on horizontal transmission mechanism between host insects and parasitoids.
出处
《中国植保导刊》
北大核心
2005年第10期5-8,共4页
China Plant Protection
基金
国家十五科技攻关项目(2004BA509B04)
国家自然科学基金(30170625)
关键词
沃尔巴克氏体
苜蓿切叶蜂
金小蜂
WSP基因
水平传播机制
Wolbachia
Megachile rotundata
Pteromalus venustus
wsp gene
horizontal transmission mechanism
